绝经后女性中雌激素诱导产生的小低密度脂蛋白颗粒。

Estrogen-induced small low-density lipoprotein particles in postmenopausal women.

作者信息

Wakatsuki A, Ikenoue N, Sagara Y

机构信息

Department of Obstetrics and Gynecology, Kochi Medical School, Japan.

出版信息

Obstet Gynecol. 1998 Feb;91(2):234-40. doi: 10.1016/s0029-7844(97)00651-0.

DOI:10.1016/s0029-7844(97)00651-0

PMID:9469282

Abstract

OBJECTIVE

To investigate the mechanisms of an estrogen-induced decrease in the size of low-density lipoprotein (LDL) particles in postmenopausal women.

METHODS

Twenty postmenopausal women were treated with conjugated equine estrogen, 0.625 mg daily, for 3 months. Plasma levels of total cholesterol, triglyceride, high-density lipoprotein (HDL) cholesterol, and apolipoproteins AI, AII, and B were measured before and after therapy. We analyzed total, free, and esterified cholesterol, triglyceride, phospholipid, and apolipoprotein B levels in the LDL. Cholesterol, triglyceride, and phospholipid concentrations were measured by enzymatic methods. Apolipoprotein AI, AII, and B levels were determined by immunoturbidimetric assay. The diameter of LDL particles was determined by gradient gel electrophoresis.

RESULTS

Estrogen reduced significantly the plasma levels of total cholesterol and apolipoprotein B and increased significantly the plasma levels of triglyceride, HDL cholesterol, and apolipoproteins AI and AII. The ratio of cholesteryl ester to apolipoprotein B was significantly reduced, whereas the ratio of triglyceride to apolipoprotein B was significantly increased after such treatment. The plasma level of triglyceride showed a positive correlation with the ratio of LDL-triglyceride/apolipoprotein B (r = .40, P < .01), and a negative correlation with the ratio of LDL-cholesteryl ester/apolipoprotein B (r = -.55, P < .001). Estrogen treatment reduced significantly the diameter of LDL particles (25.79+/-1.13 nm versus 24.94+/-1.02 nm, P < .001). The diameter of the LDL particle was correlated negatively with the plasma level of triglyceride (r = -.84, P < .001) and the ratio of LDL-triglyceride/apolipoprotein B (r = -.58, P < .001), and positively with the ratio of LDL-cholesteryl ester/apolipoprotein B (r = .57, P < .001).

CONCLUSION

The results of this study indicate that an increase in the triglyceride plasma level induced by estrogen therapy appeared to produce small triglyceride-rich and cholesteryl ester-poor LDL particles that were of small size.

摘要

目的

研究雌激素导致绝经后女性低密度脂蛋白（LDL）颗粒大小减小的机制。

方法

20名绝经后女性每日服用0.625mg结合马雌激素，持续3个月。在治疗前后测量血浆总胆固醇、甘油三酯、高密度脂蛋白（HDL）胆固醇以及载脂蛋白AI、AII和B的水平。我们分析了LDL中总胆固醇、游离胆固醇和酯化胆固醇、甘油三酯、磷脂以及载脂蛋白B的水平。胆固醇、甘油三酯和磷脂浓度通过酶法测量。载脂蛋白AI、AII和B水平通过免疫比浊法测定。LDL颗粒直径通过梯度凝胶电泳测定。

结果

雌激素显著降低了血浆总胆固醇和载脂蛋白B水平，并显著提高了血浆甘油三酯、HDL胆固醇以及载脂蛋白AI和AII水平。治疗后，胆固醇酯与载脂蛋白B的比值显著降低，而甘油三酯与载脂蛋白B的比值显著升高。血浆甘油三酯水平与LDL-甘油三酯/载脂蛋白B的比值呈正相关（r = 0.40，P < 0.01），与LDL-胆固醇酯/载脂蛋白B的比值呈负相关（r = -0.55，P < 0.001）。雌激素治疗显著减小了LDL颗粒的直径（25.79±1.13nm对24.94±1.02nm，P < 0.001）。LDL颗粒直径与血浆甘油三酯水平呈负相关（r = -0.84，P < 0.001）以及与LDL-甘油三酯/载脂蛋白B的比值呈负相关（r = -0.58，P < 0.001），与LDL-胆固醇酯/载脂蛋白B的比值呈正相关（r = 0.57，P < 0.001）。