兔体内具有青霉噻唑特异性的亲同种细胞抗体的形成。

Formation of homocytotropic antibodies with penicilloyl specificity in the rabbit.

作者信息

Rodell A K, Ahlstedt S, Olsson T K, Svärd P O

出版信息

Int Arch Allergy Appl Immunol. 1976;51(2):156-67. doi: 10.1159/000231588.

Abstract

The formation of homocytotropic antibodies (IgE) as determined by immunochemical characteristics against penicilloyl in rabbits was shown. The production of such antibodies to azidocilloyl-human serum albumin (AzO10-HSA) in alum was found optimal using 1 mg of antigen in the tested range of 0.01-8 mg. At the lowest dose (0.01 mg) only haemagglutinating but no IgE antibodies were formed. Immunization with azidocilloyl-bovine gamma-globulin (AzO9-BGG) resulted in a slower increase in antibody levels than was caused by AzO10-HSA. Univalent benzylpenicilloyl-epsilon-aminocaproate or ampicillin given together with the antigen upon immunization decreased the levels of penicllloyl specific IgE and haemagglutinating antibodies, but induced the formation of IgE antibodies against the carrier molecule. Further, the administration of penicilloyl specific IgG antibodies diminished formation of both IgE and haemagglutinating antibodies, but no antibodies specific for the carrier were formed. The usefulness of this animal model for the experimental study of penicillin allergy is discussed.

摘要

结果显示，通过对兔体内青霉噻唑基的免疫化学特性测定，证实了亲同种细胞抗体（IgE）的形成。在0.01 - 8mg的测试范围内，发现使用1mg叠氮青霉噻唑基 - 人血清白蛋白（AzO10 - HSA）与明矾一起使用时，此类抗体的产生最为理想。在最低剂量（0.01mg）时，仅形成血凝抗体，未形成IgE抗体。用叠氮青霉噻唑基 - 牛γ球蛋白（AzO9 - BGG）免疫导致抗体水平的升高比AzO10 - HSA引起的升高更为缓慢。在免疫时，将单价苄青霉素噻唑基 - ε - 氨基己酸或氨苄青霉素与抗原一起给予，可降低青霉噻唑基特异性IgE和血凝抗体的水平，但诱导了针对载体分子的IgE抗体的形成。此外，给予青霉噻唑基特异性IgG抗体可减少IgE和血凝抗体的形成，但未形成针对载体的特异性抗体。本文讨论了该动物模型在青霉素过敏实验研究中的实用性。