Keinänen R, Miettinen S, Yrjänheikki J, Koistinaho J
A.I. Virtanen Institute, University of Kuopio, Finland.
Neurosci Lett. 1997 Dec 19;239(2-3):69-72. doi: 10.1016/s0304-3940(97)00890-2.
We used in situ hybridization to localize the long-term changes in ornithine decarboxylase (ODC) expression after a 90 min occlusion of the middle cerebral artery (MCAO) in the rat. The ODC mRNA was induced in the ipsilateral dentate gyrus (DG) and throughout the ischemic cortex at 12 h and still at 3 days after reperfusion. The induction was blocked by an N-methyl-D-aspartate (NMDA) receptor antagonist suggesting that ODC induction is NMDA receptor-mediated. The long-lasting up-regulation detected in regions where no cellular damage usually occurs, favors the hypothesis that ODC expression does not contribute to neuronal death after stroke.
我们采用原位杂交技术来定位大鼠大脑中动脉闭塞（MCAO）90分钟后鸟氨酸脱羧酶（ODC）表达的长期变化。再灌注12小时及3天后，同侧齿状回（DG）和整个缺血皮层均诱导出ODC mRNA。N-甲基-D-天冬氨酸（NMDA）受体拮抗剂可阻断这种诱导，提示ODC的诱导是由NMDA受体介导的。在通常不发生细胞损伤的区域检测到的长期上调，支持了ODC表达在中风后不会导致神经元死亡的假说。
