Yu H, Peters J M, King R W, Page A M, Hieter P, Kirschner M W
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Science. 1998 Feb 20;279(5354):1219-22. doi: 10.1126/science.279.5354.1219.
The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratrico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.
后期促进复合物由八个蛋白质亚基组成，包括BimE（APC1）、CDC27（APC3）、CDC16（APC6）和CDC23（APC8）。其余四个人类APC亚基，即APC2、APC4、APC5和APC7，以及人类CDC23，已被克隆。APC7含有多个四肽重复序列拷贝，类似于CDC16、CDC23和CDC27。虽然APC4和APC5与已知功能的蛋白质没有相似性，但APC2含有一个与cullins家族中一个序列相似的区域，cullins家族的蛋白质与G1期细胞周期蛋白和细胞周期蛋白依赖性激酶抑制剂的泛素化有关。APC2基因在酿酒酵母中是必需的，apc2突变体在中期停滞，并且在Pds1p的降解方面存在缺陷。APC2和cullins可能是泛素连接酶家族的远亲成员，该家族将细胞周期调节因子作为降解目标。
