Severini C, Romi R, Zamburlini R, Di Pardo V, Marinucci M, Pierdominici G
Laboratorio di Parassitologia, Istituto Superiore di Sanità, Roma.
Ann Ist Super Sanita. 1997;33(2):245-9.
After a short review on insecticide resistance in mosquitoes, the results of a study on organophosphate (OP) resistance, carried out on 2 Culex pipiens L. field populations from North-eastern Italy, are reported. The A1 an A4-B4 or A5-B5 esterases were detected in our sample by starch gel electrophoresis. In order to differentiate between A4-B4 and A5-B5 esterases, restriction fragment length polymorphism (RFLP) analysis at the esterases B locus was performed: both A4-B4 and A5-B5 aplotipes were found in one of the studied populations. This is the first record of A5-B5 esterases in continental Europe.
在对蚊子的杀虫剂抗性进行简短回顾之后,报告了一项针对来自意大利东北部的两个尖音库蚊野外种群进行的有机磷酸酯(OP)抗性研究结果。通过淀粉凝胶电泳在我们的样本中检测到了A1以及A4 - B4或A5 - B5酯酶。为了区分A4 - B4和A5 - B5酯酶,在酯酶B位点进行了限制性片段长度多态性(RFLP)分析:在其中一个研究种群中发现了A4 - B4和A5 - B5两种单倍型。这是A5 - B5酯酶在欧洲大陆的首次记录。