Li H, He J, Lee S, Quan C, Ding J
Department of Respiratory Internal Medicine, Affiliated Hospital of Guangdong Medical College, Zhanjiang, People's Republic of China.
Ann Ist Super Sanita. 1997;33(2):273-7.
The role of phospholipase A2 (PLA2) and related inflammatory mediators in the mechanism of hypoxic pulmonary arterial hypertension was studied. Thirty Sprague-Dawley rats were equally divided into three groups at random: group A was the normal control group; group B was given 10% oxygen ventilation; group C was given 10% oxygen ventilation too and, at the same time, dexamethasone was injected into the external jugular vein. The pulmonary arterial pressure (PAP) was measured by inserting a microcatheter into pulmonary artery. After 30 minutes of hypoxia, the activity of PLA2, platelet activating factor (PAF), prostaglandin E2 (PGE2) and tromboxane B2 (TXB2) were measured in blood and lung tissue. After hypoxia, the mean pulmonary arterial pressure (mPAP), the PLA2 activity, PGE2, TXB2 and PAF in blood and lung tissue of group B were significantly higher than in group A; in group C the same parameters were lower than in group B. In hypoxia, a positive correlations was found between the PLA2 activity and mPAP, PAF, PGE2, TXB2 respectively; positive correlations were also found between PAF, PGE2, TXB2 and mPAP. PLA2 induced release of inflammation mediators, plays an important role in the mechanism of the acute hypoxic pulmonary arterial hypertension.
研究了磷脂酶A2（PLA2）及相关炎症介质在低氧性肺动脉高压机制中的作用。将30只Sprague-Dawley大鼠随机均分为三组：A组为正常对照组；B组给予10%氧气通气；C组也给予10%氧气通气，同时经颈外静脉注射地塞米松。通过将微导管插入肺动脉测量肺动脉压（PAP）。低氧30分钟后，检测血液和肺组织中PLA2、血小板活化因子（PAF）、前列腺素E2（PGE2）和血栓素B2（TXB2）的活性。低氧后，B组血液和肺组织中的平均肺动脉压（mPAP）、PLA2活性、PGE2、TXB2和PAF均显著高于A组；C组相同参数低于B组。在低氧状态下，PLA2活性分别与mPAP、PAF、PGE2、TXB2呈正相关；PAF、PGE2、TXB2与mPAP之间也呈正相关。PLA2诱导炎症介质释放，在急性低氧性肺动脉高压机制中起重要作用。
