周围神经再生：层粘连蛋白与酸性成纤维细胞生长因子的比较
Peripheral nerve regeneration: comparison of laminin and acidic fibroblast growth factor.
作者信息
Trigg D J, O'Grady K M, Bhattacharyya T, Reinke M, Toriumi D M
机构信息
Department of Otolaryngology-Head and Neck Surgery, University of Illinois at Chicago, College of Medicine, IL, USA.
出版信息
Am J Otolaryngol. 1998 Jan-Feb;19(1):29-32. doi: 10.1016/s0196-0709(98)90062-x.
PURPOSE
In an effort to show the differences between neurotrophic factors, laminin and acidic fibroblast growth factor (aFGF) were compared in terms of their abilities to regenerate axons in vivo over an extended distance.
MATERIALS AND METHODS
The sciatic nerve was transected in 15 Sprague-Dawley rats. A 15-mm Silastic tube (Dow Corning, Midland, MI) was placed between the ends of the cut nerve and filled with either laminin, aFGF, or buffer applied to collagen sponges.
RESULTS
Ten weeks postimplantation, mean axon counts showed that both laminin (2432) and aFGF (1612) produced significantly higher numbers of axons than controls (1009) (P < .05) and that laminin showed significantly more nerve regeneration than aFGF (P < .05).
CONCLUSION
These results indicate that laminin and aFGF enhance peripheral nerve regeneration across a large gap, presumably through their neurotrophic effects and mitogenic properties on supporting cells. Furthermore, it is concluded that the transient nature of aFGF's effect on the regenerative environment limits its effectiveness at regenerating axons over a prolonged period of time.
目的
为了展示神经营养因子之间的差异，对层粘连蛋白和酸性成纤维细胞生长因子（aFGF）在体内长距离轴突再生能力方面进行了比较。
材料与方法
在15只Sprague-Dawley大鼠中切断坐骨神经。将一根15毫米的硅橡胶管（道康宁公司，密歇根州米德兰）置于切断神经的两端之间，并填充层粘连蛋白、aFGF或应用于胶原海绵的缓冲液。
结果
植入后10周，平均轴突计数显示，层粘连蛋白（2432）和aFGF（1612）产生的轴突数量均显著高于对照组（1009）（P <.05），且层粘连蛋白显示出比aFGF显著更多的神经再生（P <.05）。
结论
这些结果表明，层粘连蛋白和aFGF可促进大间隙周围神经的再生，推测是通过它们对支持细胞的神经营养作用和促有丝分裂特性。此外，得出结论，aFGF对再生环境影响的短暂性限制了其在长时间轴突再生中的有效性。
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