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通过聚丙烯酰胺凝胶电泳分离犬催乳素。

Isolation of canine prolactin by polyacrylamide gel electrophoresis.

作者信息

Jones G E, Brownstone A D, Boyns A R

出版信息

Acta Endocrinol (Copenh). 1976 Aug;82(4):691-705. doi: 10.1530/acta.0.0820691.

DOI:10.1530/acta.0.0820691
PMID:947280
Abstract

The electrophoretic mobility of prolactin obtained from canine pituitary extract was studied with the aid of polyacrylamide disc electrophoresis. Using a preparative gel electrophoretic system the immunoreactive material was purified on a quantitative scale which was then used to develop a homologous radioimmunoassay for canine prolactin. The radioimmunoassay system was able to detect prolactin in the plasma of dogs after the administration of agents which would be expected to affect prolactin secretion.

摘要

借助聚丙烯酰胺圆盘电泳研究了从犬垂体提取物中获得的催乳素的电泳迁移率。使用制备性凝胶电泳系统对免疫反应性物质进行了定量纯化,然后用于开发犬催乳素的同源放射免疫测定法。该放射免疫测定系统能够在给予预期会影响催乳素分泌的药物后检测犬血浆中的催乳素。

相似文献

1
Isolation of canine prolactin by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳分离犬催乳素。
Acta Endocrinol (Copenh). 1976 Aug;82(4):691-705. doi: 10.1530/acta.0.0820691.
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Purification of canine prolactin by preparative isotachophoresis.通过制备性等速电泳法纯化犬催乳素。
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Electrophoretic separation of prolactin secreted from mouse pituitary glands in vitro.
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Homologous radioimmunoassay for canine prolactin and its application in various physiological states.犬催乳素的同源放射免疫测定及其在各种生理状态下的应用。
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Electrophoretic identity of biologically active pituitary and serum prolactins in the lactating and perphenazine-treated rat.哺乳期及经奋乃静处理的大鼠体内生物活性垂体催乳素和血清催乳素的电泳同一性
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Isolation of canine prolactin by anterior pituitary organ culture.通过垂体前叶器官培养分离犬催乳素。
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Measurement of canine pituitary prolactin.犬垂体催乳素的测量。
J Endocrinol. 1973 Feb;56(2):245-58. doi: 10.1677/joe.0.0560245.

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