缺乏单链核酸外切酶ExoI、ExoVII和RecJ的大肠杆菌细胞中的错配修复
Mismatch repair in Escherichia coli cells lacking single-strand exonucleases ExoI, ExoVII, and RecJ.
作者信息
Harris R S, Ross K J, Lombardo M J, Rosenberg S M
机构信息
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
出版信息
J Bacteriol. 1998 Feb;180(4):989-93. doi: 10.1128/JB.180.4.989-993.1998.
Abstract
In vitro, the methyl-directed mismatch repair system of Escherichia coli requires the single-strand exonuclease activity of either ExoI, ExoVII, or RecJ and possibly a fourth, unknown single-strand exonuclease. We have created the first precise null mutations in genes encoding ExoI and ExoVII and find that cells lacking these nucleases and RecJ perform mismatch repair in vivo normally such that triple-null mutants display normal mutation rates. ExoI, ExoVII, and RecJ are either redundant with another function(s) or are unnecessary for mismatch repair in vivo.
摘要
在体外，大肠杆菌的甲基导向错配修复系统需要ExoI、ExoVII或RecJ的单链核酸外切酶活性，可能还需要第四种未知的单链核酸外切酶。我们在编码ExoI和ExoVII的基因中创建了首个精确的无效突变，发现缺乏这些核酸酶和RecJ的细胞在体内仍能正常进行错配修复，因此三基因无效突变体显示出正常的突变率。ExoI、ExoVII和RecJ要么与其他功能冗余，要么在体内错配修复中并非必需。
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