酿酒酵母EXO1的鉴定与特性分析，EXO1是一个编码与MSH2相互作用的核酸外切酶的基因。
Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2.
作者信息
Tishkoff D X, Boerger A L, Bertrand P, Filosi N, Gaida G M, Kane M F, Kolodner R D
机构信息
Division of Human Cancer Genetics, Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7487-92. doi: 10.1073/pnas.94.14.7487.
Abstract
A two-hybrid screen was used to identify Saccharomyces cerevisiae genes encoding proteins that interact with MSH2. One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5'-3' exonuclease. S. cerevisiae EXO1 interacted with both S. cerevisiae and human MSH2 in two-hybrid and coimmunoprecipitation experiments. exo1 mutants showed a mutator phenotype, and epistasis analysis was consistent with EXO1 functioning in the MSH2-dependent mismatch repair pathway. exo1 mutations were lethal in combination with rad27 mutations, and overexpression of EXO1 suppressed both the temperature sensitive and mutator phenotypes of rad27 mutants.
摘要
利用双杂交筛选来鉴定酿酒酵母中编码与MSH2相互作用蛋白的基因。发现一个基因编码粟酒裂殖酵母EXO1的同源物，EXO1是一种双链DNA特异性5'-3'核酸外切酶。在双杂交和免疫共沉淀实验中，酿酒酵母EXO1与酿酒酵母和人类MSH2都相互作用。exo1突变体表现出突变体表型，上位性分析表明EXO1在MSH2依赖的错配修复途径中发挥作用。exo1突变与rad27突变组合是致死的，EXO1的过表达抑制了rad27突变体的温度敏感性和突变体表型。
相似文献
1
Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2.酿酒酵母EXO1的鉴定与特性分析，EXO1是一个编码与MSH2相互作用的核酸外切酶的基因。
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7487-92. doi: 10.1073/pnas.94.14.7487.
2
Saccharomyces cerevisiae exonuclease-1 plays a role in UV resistance that is distinct from nucleotide excision repair.酿酒酵母核酸外切酶-1在紫外线抗性中发挥作用，这一作用与核苷酸切除修复不同。
Nucleic Acids Res. 1998 Jul 1;26(13):3077-83. doi: 10.1093/nar/26.13.3077.
3
EXO1 and MSH6 are high-copy suppressors of conditional mutations in the MSH2 mismatch repair gene of Saccharomyces cerevisiae.EXO1和MSH6是酿酒酵母MSH2错配修复基因中条件性突变的高拷贝抑制因子。
Genetics. 2000 Jun;155(2):589-99. doi: 10.1093/genetics/155.2.589.
4
5
The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae.DNA聚合酶δ和ε的3'→5'核酸外切酶以及5'→3'核酸外切酶Exo1在酿酒酵母复制后避免突变中起主要作用。
Mol Cell Biol. 1999 Mar;19(3):2000-7. doi: 10.1128/MCB.19.3.2000.
6
Schizosaccharomyces pombe exo1 is involved in the same mismatch repair pathway as msh2 and pms1.粟酒裂殖酵母exo1与msh2和pms1参与相同的错配修复途径。
Curr Genet. 1998 Dec;34(5):343-50. doi: 10.1007/s002940050405.
7
Identification of Exo1-Msh2 interaction motifs in DNA mismatch repair and new Msh2-binding partners.鉴定 DNA 错配修复中外切酶 1-Msh2 相互作用基序和新的 Msh2 结合伴侣。
Nat Struct Mol Biol. 2018 Aug;25(8):650-659. doi: 10.1038/s41594-018-0092-y. Epub 2018 Jul 30.
8
Reconstitution of DNA polymerase ε-dependent mismatch repair with purified proteins.用纯化蛋白重建依赖于 DNA 聚合酶 ε 的错配修复。
Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):3607-3612. doi: 10.1073/pnas.1701753114. Epub 2017 Mar 6.
9
Rad27 and Exo1 function in different excision pathways for mismatch repair in Saccharomyces cerevisiae.Rad27 和 Exo1 在酿酒酵母错配修复的不同切除途径中发挥作用。
Nat Commun. 2021 Sep 22;12(1):5568. doi: 10.1038/s41467-021-25866-z.
10
Complementary functions of the Saccharomyces cerevisiae Rad2 family nucleases in Okazaki fragment maturation, mutation avoidance, and chromosome stability.酿酒酵母Rad2家族核酸酶在冈崎片段成熟、突变避免和染色体稳定性中的互补功能。
DNA Repair (Amst). 2003 Aug 12;2(8):925-40. doi: 10.1016/s1568-7864(03)00093-4.
引用本文的文献
1
DNA lesions can frequently precede DNA:RNA hybrid accumulation.DNA损伤常常先于DNA:RNA杂交体的积累出现。
Nat Commun. 2025 Mar 10;16(1):2401. doi: 10.1038/s41467-025-57588-x.
2
Action-At-A-Distance in DNA Mismatch Repair: Mechanistic Insights and Models for How DNA and Repair Proteins Facilitate Long-Range Communication.远距离作用在 DNA 错配修复中的作用：DNA 和修复蛋白如何促进长程通讯的机制见解和模型。
Biomolecules. 2024 Nov 13;14(11):1442. doi: 10.3390/biom14111442.
3
S-phase checkpoint prevents leading strand degradation from strand-associated nicks at stalled replication forks.S 期检查点防止停滞复制叉处链相关缺口导致的前导链降解。
Nucleic Acids Res. 2024 May 22;52(9):5121-5137. doi: 10.1093/nar/gkae192.
4
MRE11A: a novel negative regulator of human DNA mismatch repair.MRE11A：一种新型的人 DNA 错配修复负调控因子。
Cell Mol Biol Lett. 2024 Mar 14;29(1):37. doi: 10.1186/s11658-024-00547-z.
5
Elevated MSH2 MSH3 expression interferes with DNA metabolism in vivo.MSH2 MSH3 表达水平升高会干扰体内的 DNA 代谢。
Nucleic Acids Res. 2023 Dec 11;51(22):12185-12206. doi: 10.1093/nar/gkad934.
6
Exo1 protects DNA nicks from ligation to promote crossover formation during meiosis.Exo1 保护 DNA 切口免受连接，以促进减数分裂过程中的交叉形成。
PLoS Biol. 2023 Apr 20;21(4):e3002085. doi: 10.1371/journal.pbio.3002085. eCollection 2023 Apr.
7
Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombination.解旋酶 Rad27 可切割 R 环结构的 RNA 以抑制端粒重组。
Nucleic Acids Res. 2023 May 22;51(9):4398-4414. doi: 10.1093/nar/gkad236.
8
Ataxia Telangiectasia Mutated and MSH2 Control Blunt DNA End Joining in Ig Class Switch Recombination.共济失调毛细血管扩张症突变基因和 MSH2 控制免疫球蛋白类别转换重组中 DNA 末端连接的钝性。
J Immunol. 2023 Feb 15;210(4):369-376. doi: 10.4049/jimmunol.2200590.
9
as a Model System for Eukaryotic Cell Biology, from Cell Cycle Control to DNA Damage Response.作为真核细胞生物学的模式系统，从细胞周期调控到 DNA 损伤反应。
Int J Mol Sci. 2022 Oct 1;23(19):11665. doi: 10.3390/ijms231911665.
10
Role of EXO1 nuclease activity in genome maintenance, the immune response and tumor suppression in Exo1D173A mice.EXO1 核酸酶活性在基因组维持、免疫反应和 Exo1D173A 小鼠肿瘤抑制中的作用。
Nucleic Acids Res. 2022 Aug 12;50(14):8093-8106. doi: 10.1093/nar/gkac616.
本文引用的文献
1
Exonuclease I of Saccharomyces cerevisiae functions in mitotic recombination in vivo and in vitro.酿酒酵母的核酸外切酶 I 在体内和体外的有丝分裂重组中发挥作用。
Mol Cell Biol. 1997 May;17(5):2764-73. doi: 10.1128/MCB.17.5.2764.
2
The FEN-1 family of structure-specific nucleases in eukaryotic DNA replication, recombination and repair.真核生物DNA复制、重组和修复中结构特异性核酸酶的FEN-1家族。
Bioessays. 1997 Mar;19(3):233-40. doi: 10.1002/bies.950190309.
3
Characterization of a novel DNA damage-inducible gene of Saccharomyces cerevisiae, DIN7, which is a structural homolog of the RAD2 and RAD27 DNA repair genes.酿酒酵母一种新的DNA损伤诱导基因DIN7的特性分析，该基因是RAD2和RAD27 DNA修复基因的结构同源物。
Mol Gen Genet. 1997 Feb 27;253(6):655-65. doi: 10.1007/s004380050369.
4
Saccharomyces cerevisiae MSH2, a mispaired base recognition protein, also recognizes Holliday junctions in DNA.酿酒酵母MSH2是一种错配碱基识别蛋白，它也能识别DNA中的霍利迪连接体。
J Mol Biol. 1997 Jan 24;265(3):289-301. doi: 10.1006/jmbi.1996.0743.
5
A novel mutation avoidance mechanism dependent on S. cerevisiae RAD27 is distinct from DNA mismatch repair.一种依赖酿酒酵母RAD27的新型突变避免机制不同于DNA错配修复。
Cell. 1997 Jan 24;88(2):253-63. doi: 10.1016/s0092-8674(00)81846-2.
6
Meiotic recombination in yeast: coronation of the double-strand-break repair model.酵母中的减数分裂重组：双链断裂修复模型的确立
Cell. 1996 Dec 13;87(6):965-8. doi: 10.1016/s0092-8674(00)81791-2.
7
Microsatellite instability and mutation analysis of hMSH2 and hMLH1 in patients with sporadic, familial and hereditary colorectal cancer.散发性、家族性和遗传性结直肠癌患者中hMSH2和hMLH1的微卫星不稳定性及突变分析
Hum Mol Genet. 1996 Sep;5(9):1245-52. doi: 10.1093/hmg/5.9.1245.
8
Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae.酿酒酵母有丝分裂重组RAD1-RAD10途径中错配修复基因MSH2和MSH3的需求。
Genetics. 1996 Mar;142(3):727-36. doi: 10.1093/genetics/142.3.727.
9
Tosca: a Drosophila gene encoding a nuclease specifically expressed in the female germline.托斯卡：一个果蝇基因，编码一种在雌性生殖系中特异性表达的核酸酶。
Dev Biol. 1996 Aug 25;178(1):90-100. doi: 10.1006/dbio.1996.0200.
10
Biochemistry and genetics of eukaryotic mismatch repair.真核生物错配修复的生物化学与遗传学
Genes Dev. 1996 Jun 15;10(12):1433-42. doi: 10.1101/gad.10.12.1433.
Zotero 插件