反义env寡脱氧核苷酸对人1型T细胞白血病病毒复制的抑制作用

Inhibition of human T-cell leukemia virus type 1 replication by antisense env oligodeoxynucleotide.

作者信息

Maeda N, Kawamura T, Hoshino H, Yamada N, Blackard J, Kushida S, Miyano-Kurosaki N, Yamamoto N, Makino K, Yokota T, Uchida K, Miwa M

机构信息

Department of Biochemistry and Molecular Oncology, University of Tsukuba, Japan.

出版信息

Biochem Biophys Res Commun. 1998 Feb 4;243(1):109-12. doi: 10.1006/bbrc.1997.8039.

DOI:10.1006/bbrc.1997.8039

PMID:9473488

Abstract

Human T-cell leukemia virus type 1 (HTLV-1) infection is associated with adult T-cell leukemia and HTLV-associated myelopathy/tropical spastic paraparesis. Inhibition of HTLV-1 transmission is important to prevent the above HTLV-1-associated diseases. We used the antisense oligodeoxynucleotides (oligos) complementary to the first splice junction, rex responsive site, gag, env, tax, rex, and p21 and evaluated the effects on the syncytium formation between HTLV-1 producing human T-cell line, C9/PL cells, and HTLV-1-uninfected human glioma cell line, U251-MG cells. The syncytium formation was significantly inhibited the virion production assayed by antisense oligos to env, tax, gag, p21, and rex, with antisense oligo to env being the most inhibitory. Antisense oligos to env and tax also inhibited reverse transcriptase activity. Antisense oligo to env may have a potential as a preventive measure of HTLV-1 replication and transmission in vivo.

摘要

人类嗜T淋巴细胞病毒1型（HTLV-1）感染与成人T细胞白血病及HTLV相关脊髓病/热带痉挛性截瘫有关。抑制HTLV-1传播对于预防上述HTLV-1相关疾病至关重要。我们使用了与第一个剪接连接点、rex反应位点、gag、env、tax、rex和p21互补的反义寡脱氧核苷酸（oligos），并评估了其对产生HTLV-1的人T细胞系C9/PL细胞与未感染HTLV-1的人胶质瘤细胞系U251-MG细胞之间合胞体形成的影响。针对env、tax、gag、p21和rex的反义oligos显著抑制了合胞体形成，其中针对env的反义oligo抑制作用最强。针对env和tax的反义oligos也抑制了逆转录酶活性。针对env的反义oligo可能具有作为体内预防HTLV-1复制和传播措施的潜力。