一种人胃癌细胞系拥有胃泌素释放肽的功能性受体。

A human gastric cancer cell line possesses a functional receptor for gastrin-releasing peptide.

作者信息

Bold R J, Kim H J, Ishizuka J, Townsend C M, Thompson J C

机构信息

Department of Surgery, University of California, Davis-East Bay, Oakland 94602, USA.

出版信息

Cancer Invest. 1998;16(1):12-7. doi: 10.3109/07357909809039748.

DOI:10.3109/07357909809039748

PMID:9474246

Abstract

Bombesin (BBS) exhibits diverse biological functions including those of neurotransmitter, regulator of gastrointestinal hormone release, and mitogen. Gastrin-releasing peptide (GRP, the mammalian equivalent of BBS) is found in mucosal cells of the gastric fundus and antrum. We determined whether a human gastric cancer cell line (SIIA) expresses a functional GRP-receptor (GRP-R). BBS increased intracellular calcium ([Ca2+]i), and a specific GRP-R antagonist, ([D-Phe6, Des-Met14]-BBS (6-14)-ethylamide), blocked BBS-induced increase in [Ca2+]i. SIIA cells possess GRP-R mRNA by reverse transcriptase-PCR. Furthermore, these cells possess an 80-kDa cell surface protein that specifically binds BBS with two high-binding affinities (Kd1 = 0.6 nM, Kd2 = 6.7 nM). These findings indicate that SIIA cells possess a GRP-R that is capable of physiological signal transduction, though the cellular response remains unknown.

摘要

蛙皮素（BBS）具有多种生物学功能，包括作为神经递质、胃肠激素释放调节剂和促细胞分裂剂。胃泌素释放肽（GRP，BBS的哺乳动物等同物）存在于胃底和胃窦的黏膜细胞中。我们确定了一种人胃癌细胞系（SIIA）是否表达功能性胃泌素释放肽受体（GRP-R）。蛙皮素增加细胞内钙浓度（[Ca2+]i），而一种特异性胃泌素释放肽受体拮抗剂（[D-苯丙氨酸6，去甲蛋氨酸14]-蛙皮素（6-14）乙酰胺）可阻断蛙皮素诱导的[Ca2+]i增加。通过逆转录聚合酶链反应（RT-PCR）检测发现SIIA细胞含有胃泌素释放肽受体信使核糖核酸（GRP-R mRNA）。此外，这些细胞具有一种80 kDa的细胞表面蛋白，该蛋白以两种高结合亲和力（Kd1 = 0.6 nM，Kd2 = 6.7 nM）特异性结合蛙皮素。这些发现表明，SIIA细胞拥有一种能够进行生理信号转导的胃泌素释放肽受体，尽管细胞反应尚不清楚。