Akisue T, Jinnai H, Hitomi T, Miwa N, Yoshida K, Nakamura S
Department of Biochemistry, Kobe University School of Medicine, Japan.
FEBS Lett. 1998 Jan 23;422(1):108-12. doi: 10.1016/s0014-5793(97)01611-6.
A heat-stable activator for ADP-ribosylation factor (ARF)-dependent phospholipase D (PLD) was purified to near homogeneity from rat kidney cytosol by a sequential column chromatography. The purified activator has a molecular mass of 23 kDa on SDS-PAGE. Using a partially purified ARF-dependent PLD from rat kidney, the activator synergistically stimulates PLD with ARF in time- and dose-dependent manner. In the absence of ARF, the activator has little or no effect. The purified activator also stimulates PLD under several conditions including permeabilized cell system, suggesting that the activator is a physiologically relevant regulator of PLD.
通过连续柱色谱法从大鼠肾细胞溶质中纯化出一种热稳定的、用于ADP核糖基化因子（ARF）依赖性磷脂酶D（PLD）的激活剂，使其纯度接近均一。纯化后的激活剂在SDS-PAGE上的分子量为23 kDa。使用从大鼠肾中部分纯化的ARF依赖性PLD，该激活剂能以时间和剂量依赖性方式与ARF协同刺激PLD。在没有ARF的情况下，激活剂几乎没有作用或没有作用。纯化后的激活剂在包括透化细胞系统在内的几种条件下也能刺激PLD，这表明该激活剂是PLD的一种生理相关调节因子。
