Figures W R, Edwards J R
Carbohydr Res. 1976 Jun;48(2):245-53. doi: 10.1016/s0008-6215(00)83220-6.
alpha-D-glucopyranosyl fluoride can serve as the D-glucopyranosyl donor for the glycosyltransferase from Streptococcus mutans. The products of the reaction are a D-glucan of high molecular weight and fluoride ion. The rate of reaction was measured by an electrode specific for the fluoride ion. The reaction was inhibited by the substrate at concentrations greater than 30 mM, but was not affected by fluoride ion. There was little inhibition of the reaction by a series of monosaccharides, except for 2-amino-2-deoxy-D-glucose, D-mannitol, and 3-deoxy-D-ribo-hexose. Maltose, isomaltose, isomaltotriose, and D-fructose all stimulated the release of fluoride from alpha-D-glucopyranosyl fluoride.
α-D-吡喃葡萄糖基氟化物可作为变形链球菌糖基转移酶的D-吡喃葡萄糖基供体。反应产物是高分子量的D-葡聚糖和氟离子。反应速率通过对氟离子具有特异性的电极进行测定。当底物浓度大于30 mM时，反应受到抑制，但不受氟离子影响。除2-氨基-2-脱氧-D-葡萄糖、D-甘露醇和3-脱氧-D-核糖己糖外，一系列单糖对该反应几乎没有抑制作用。麦芽糖、异麦芽糖、异麦芽三糖和D-果糖均能促进α-D-吡喃葡萄糖基氟化物释放氟离子。
