Mallin D R, Myung J S, Patton J S, Geyer P K
Department of Biochemistry, The University of Iowa, Iowa City 52242, USA.
Genetics. 1998 Jan;148(1):331-9. doi: 10.1093/genetics/148.1.331.
The suppressor of Hairy-wing [SU(HW)] binding region disrupts communication between a large number of enhancers and promoters and protects transgenes from chromosomal position effects. These properties classify the SU(HW) binding region as an insulator. While enhancers are blocked in a general manner, protection from repressors appears to be more variable. In these studies, we address whether repression resulting from the Polycomb group genes can be blocked by the SU(HW) binding region. The effects of this binding region on repression established by an Ultrabithorax Polycomb group Response Element were examined. A transposon carrying two reporter genes, the yellow and white genes, was used so that repression and insulation could be assayed simultaneously. We demonstrate that the SU(HW) binding region is effective at preventing Polycomb group repression. These studies suggest that one role of the su(Hw) protein may be to restrict the range of action of repressors, such as the Polycomb group proteins, throughout the euchromatic regions of the genome.
毛翅抑制因子[SU(HW)]结合区域破坏了大量增强子与启动子之间的通讯，并保护转基因免受染色体位置效应的影响。这些特性将SU(HW)结合区域归类为绝缘子。虽然增强子通常被阻断，但对阻遏物的保护作用似乎更具变异性。在这些研究中，我们探讨了多梳蛋白家族基因导致的抑制作用是否能被SU(HW)结合区域阻断。研究了该结合区域对由超双胸节多梳蛋白家族反应元件建立的抑制作用的影响。使用携带两个报告基因（黄色和白色基因）的转座子，以便同时检测抑制作用和绝缘作用。我们证明SU(HW)结合区域在防止多梳蛋白家族抑制方面是有效的。这些研究表明，su(Hw)蛋白的一个作用可能是限制阻遏物（如多梳蛋白家族蛋白）在整个基因组常染色质区域的作用范围。
