Pedersen K E, Meeker S N, Riccio M M, Undem B J
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA.
J Appl Physiol (1985). 1998 Feb;84(2):499-506. doi: 10.1152/jappl.1998.84.2.499.
We evaluated the ability of hyperosmolar stimuli to activate afferent nerves in the guinea pig trachea and main bronchi and investigated the neural pathways involved. By using electrophysiological techniques, studies in vitro examined the effect of hyperosmolar solutions of sodium chloride (hypertonic saline) on guinea pig airway afferent nerve endings arising from either vagal nodose or jugular ganglia. The data reveal a differential sensitivity of airway afferent neurons to activation with hypertonic saline. Afferent fibers (both A delta and C fibers) with cell bodies located in jugular ganglia were much more sensitive to stimulation with hypertonic saline, compared with afferent neurons with cell bodies located in nodose ganglia. Additional studies in vivo demonstrated that inhalation of aerosols of hypertonic saline induced plasma extravasation in guinea pig trachea that was mediated via tachykinin NK1 receptors. Identification of a differential sensitivity of guinea pig airway afferent nerves to hypertonic saline leads to the speculation that airway responses to hyperosmolar stimuli may result from activation of afferent neurons originating predominantly from the jugular ganglion.
我们评估了高渗刺激激活豚鼠气管和主支气管传入神经的能力，并研究了其中涉及的神经通路。通过使用电生理技术，体外研究检测了氯化钠高渗溶液（高渗盐水）对源自迷走神经结状神经节或颈神经节的豚鼠气道传入神经末梢的影响。数据显示气道传入神经元对高渗盐水激活具有不同的敏感性。与细胞体位于结状神经节的传入神经元相比，细胞体位于颈神经节的传入纤维（Aδ纤维和C纤维）对高渗盐水刺激更为敏感。体内的其他研究表明，吸入高渗盐水气雾剂可诱导豚鼠气管血浆外渗，这是通过速激肽NK1受体介导的。豚鼠气道传入神经对高渗盐水的不同敏感性的确定引发了这样的推测，即气道对高渗刺激的反应可能源于主要源自颈神经节的传入神经元的激活。
