卫可无泡消毒剂对乙型肝炎病毒的作用

The action of VIRKON No Foam on the hepatitis B virus.

作者信息

Scioli D, Pizzella T, Vollaro L, Nardiello S, De Feo L

机构信息

University of Naples Federico II, Department of General and Environmental Physiology, Italy.

出版信息

Eur J Epidemiol. 1997 Dec;13(8):879-83. doi: 10.1023/a:1007399926095.

DOI:10.1023/a:1007399926095

PMID:9476816

Abstract

The aim of this work was to verify the in vitro virucidal activity of VIRKON No Foam (VIRKON NF) against the HBV DNA, notoriously one the most resistant viruses to heat and to the most commonly used disinfectants. VIRKON NF, an oxidizing agent, has a synergic effect on nucleic acids, polypeptides, glycoproteins, and structural proteins. The experiment was conducted using the serum of a patient with HBsAg+/HBeAg+/HBVDNA+ with a DNA concentration 100 pg/ml. The virucidal activity was tested using the Slot Blot method, the result being evaluated as an inhibition of the autoradiographic signal because of contact of different concentrated solutions and various contact times with the serum. Preliminary tests were carried out after contact of VIRKON NF with serum solutions at 0.1-0.5-1-4% concentrations for a 15 min contact time. VIRKON NF showed an inhibition of autoradiographic signal equal respectively to 0%, 25-50%, 50-70%, 100%. Further experiments were carried out to compare VIRKON NF virucidal activity on HBV to the activity of the most common disinfectants, such as formic aldehyde, Amuchina, glutaraledhyde and phenol. In this way, the infected serum was put into contact with both the most common disinfectant and 1-1.5-2-3-4% VIRKON NF solutions in contact times of 15, 10, 5, 2 min. Using 3% concentrations for a contact time of 10 min, VIRKON NF showed an autoradiographic inhibition signal of 90%. Regarding other disinfectants, only a 2% glutaraldehyde solution for a contact time of 15 min showed similar virucidal activity.

摘要

本研究旨在验证无泡型卫康（VIRKON NF）对乙肝病毒DNA的体外杀病毒活性，乙肝病毒是出了名的对热和最常用消毒剂具有高度抗性的病毒之一。VIRKON NF是一种氧化剂，对核酸、多肽、糖蛋白和结构蛋白具有协同作用。实验使用了一名乙肝表面抗原（HBsAg）阳性/乙肝e抗原（HBeAg）阳性/乙肝病毒DNA（HBVDNA）阳性患者的血清，其DNA浓度为100 pg/ml。采用狭缝杂交法检测杀病毒活性，通过不同浓度溶液与血清在不同接触时间下的接触，以放射自显影信号的抑制情况来评估结果。在VIRKON NF与浓度为0.1%、0.5%、1%、4%的血清溶液接触15分钟后进行初步试验。VIRKON NF对放射自显影信号的抑制率分别为0%、25%-50%、50%-70%、100%。进一步开展实验，将VIRKON NF对乙肝病毒的杀病毒活性与最常用的消毒剂如甲醛、戊二醛、戊二醛和苯酚的活性进行比较。具体做法是，将感染血清分别与最常用消毒剂以及浓度为1%、1.5%、2%、3%和4%的VIRKON NF溶液接触，接触时间分别为15分钟、10分钟、5分钟和2分钟。使用3%的VIRKON NF溶液接触10分钟，其放射自显影抑制信号为90%。对于其他消毒剂，只有2%的戊二醛溶液在接触15分钟时显示出类似的杀病毒活性。