利什曼原虫（硕大利什曼原虫弗里德林株）基因组物理图谱。
A physical map of the Leishmania major Friedlin genome.
作者信息
Ivens A C, Lewis S M, Bagherzadeh A, Zhang L, Chan H M, Smith D F
机构信息
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, UK.
出版信息
Genome Res. 1998 Feb;8(2):135-45. doi: 10.1101/gr.8.2.135.
Abstract
An extensive physical map of the Leishmania major Friedlin genome has been assembled by the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization. The integrated data obtained for 9004 fingerprinted clones and 974 probes have placed 91.2% of the 33.58-Mb genome into contigs representing each of the 36 chromosomes. This first-generation map has already provided a suitable framework for both high-throughput DNA sequencing and functional studies of the L. major parasite.
摘要
通过穿梭载体黏粒文库的指纹分析与探针杂交相结合，构建了硕大利什曼原虫弗里德林株基因组的精细物理图谱。对9004个经指纹分析的克隆和974个探针获得的整合数据，已将33.58 Mb基因组的91.2%定位到代表36条染色体中每一条的重叠群中。这一第一代图谱已为硕大利什曼原虫的高通量DNA测序和功能研究提供了合适的框架。
相似文献
1
A physical map of the Leishmania major Friedlin genome.利什曼原虫（硕大利什曼原虫弗里德林株）基因组物理图谱。
Genome Res. 1998 Feb;8(2):135-45. doi: 10.1101/gr.8.2.135.
2
Parasite genome analysis. A global map of the Leishmania major genome: prelude to genomic sequencing.寄生虫基因组分析。利什曼原虫主要基因组的全球图谱：基因组测序的前奏。
Trans R Soc Trop Med Hyg. 1997 Mar-Apr;91(2):111-5. doi: 10.1016/s0035-9203(97)90188-7.
3
Shotgun optical mapping of the entire Leishmania major Friedlin genome.利什曼原虫（硕大利什曼原虫弗里德林株）全基因组的鸟枪法光学图谱分析
Mol Biochem Parasitol. 2004 Nov;138(1):97-106. doi: 10.1016/j.molbiopara.2004.08.002.
4
Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome.对七个复杂基因座进行重测序和组装以改进硕大利什曼原虫（Friedlin株）参考基因组。
Parasit Vectors. 2016 Feb 8;9:74. doi: 10.1186/s13071-016-1329-4.
5
Leishmania major: histone H1 gene expression from the sw3 locus.硕大利什曼原虫：来自sw3基因座的组蛋白H1基因表达
Exp Parasitol. 1999 Feb;91(2):151-60. doi: 10.1006/expr.1998.4378.
6
7
Recent developments from the Leishmania genome project.利什曼原虫基因组计划的最新进展。
Curr Opin Microbiol. 2000 Aug;3(4):412-6. doi: 10.1016/s1369-5274(00)00113-2.
8
Leishmania major: molecular cloning, sequencing, and expression of the heat shock protein 60 gene reveals unique carboxy terminal peptide sequences.硕大利什曼原虫：热休克蛋白60基因的分子克隆、测序及表达揭示了独特的羧基末端肽序列。
Exp Parasitol. 1997 Mar;85(3):249-63. doi: 10.1006/expr.1996.4137.
9
Evaluation of a cosmid contig physical map of human chromosome 16.人类16号染色体黏粒重叠群物理图谱的评估
Genomics. 1992 Aug;13(4):1031-9. doi: 10.1016/0888-7543(92)90016-l.
10
Gene Annotation and Transcriptome Delineation on a De Novo Genome Assembly for the Reference Friedlin Strain.从头组装的参考弗里德林（Friedlin）株的基因注释和转录组描绘。
Genes (Basel). 2021 Aug 29;12(9):1359. doi: 10.3390/genes12091359.
引用本文的文献
1
Gene Annotation and Transcriptome Delineation on a De Novo Genome Assembly for the Reference Friedlin Strain.从头组装的参考弗里德林（Friedlin）株的基因注释和转录组描绘。
Genes (Basel). 2021 Aug 29;12(9):1359. doi: 10.3390/genes12091359.
2
Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome.对七个复杂基因座进行重测序和组装以改进硕大利什曼原虫（Friedlin株）参考基因组。
Parasit Vectors. 2016 Feb 8;9:74. doi: 10.1186/s13071-016-1329-4.
3
The Alveolate Perkinsus marinus: biological insights from EST gene discovery.《具膜有孔虫目 Perkinsus marinus：EST 基因发现带来的生物学见解》。
BMC Genomics. 2010 Apr 7;11:228. doi: 10.1186/1471-2164-11-228.
4
Characterization of trifluralin binding with recombinant tubulin from Trypanosoma brucei.氟乐灵与布氏锥虫重组微管蛋白结合的特性研究
Parasitol Res. 2009 Mar;104(4):893-903. doi: 10.1007/s00436-008-1271-2. Epub 2008 Dec 3.
5
Two functionally divergent UDP-Gal nucleotide sugar transporters participate in phosphoglycan synthesis in Leishmania major.两种功能不同的UDP-半乳糖核苷酸糖转运蛋白参与了硕大利什曼原虫的磷酸聚糖合成。
J Biol Chem. 2007 May 11;282(19):14006-17. doi: 10.1074/jbc.M610869200. Epub 2007 Mar 8.
6
Differential gene expression in benznidazole-resistant Trypanosoma cruzi parasites.苯并硝唑抗性克氏锥虫寄生虫中的差异基因表达
Antimicrob Agents Chemother. 2005 Jul;49(7):2701-9. doi: 10.1128/AAC.49.7.2701-2709.2005.
7
Targeting enzymes involved in spermidine metabolism of parasitic protozoa--a possible new strategy for anti-parasitic treatment.靶向参与寄生原生动物亚精胺代谢的酶——一种抗寄生虫治疗的可能新策略。
Parasitol Res. 2003 Dec;91(6):508-16. doi: 10.1007/s00436-003-0970-y. Epub 2003 Oct 7.
8
Leishmania major chromosome 3 contains two long convergent polycistronic gene clusters separated by a tRNA gene.硕大利什曼原虫3号染色体包含两个长的反向多顺反子基因簇，中间由一个tRNA基因隔开。
Nucleic Acids Res. 2003 Jul 15;31(14):4201-10. doi: 10.1093/nar/gkg469.
9
Secretory pathway of trypanosomatid parasites.锥虫寄生虫的分泌途径。
Microbiol Mol Biol Rev. 2002 Mar;66(1):122-54; table of contents. doi: 10.1128/MMBR.66.1.122-154.2002.
10
The structure of aspartyl dipeptidase reveals a unique fold with a Ser-His-Glu catalytic triad.天冬氨酰二肽酶的结构揭示了一种具有丝氨酸-组氨酸-谷氨酸催化三联体的独特折叠。
Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14097-102. doi: 10.1073/pnas.260376797.
本文引用的文献
1
Differentially expressed Leishmania major gp63 genes encode cell surface leishmanolysin with distinct signals for glycosylphosphatidylinositol attachment.差异表达的硕大利什曼原虫gp63基因编码具有不同糖基磷脂酰肌醇附着信号的细胞表面利什曼溶素。
Mol Biochem Parasitol. 1998 May 15;93(1):31-41. doi: 10.1016/s0166-6851(98)00013-9.
2
A direct method for the chromosomal assignment of DNA markers in Leishmania.利什曼原虫DNA标记染色体定位的直接方法。
Gene. 1997 Jul 18;194(1):77-80. doi: 10.1016/s0378-1119(97)00162-5.
3
Parasite genome analysis. A global map of the Leishmania major genome: prelude to genomic sequencing.寄生虫基因组分析。利什曼原虫主要基因组的全球图谱：基因组测序的前奏。
Trans R Soc Trop Med Hyg. 1997 Mar-Apr;91(2):111-5. doi: 10.1016/s0035-9203(97)90188-7.
4
Trans-kingdom transposition of the Drosophila element mariner within the protozoan Leishmania.果蝇mariner元件在原生动物利什曼原虫中的跨界转座。
Science. 1997 Jun 13;276(5319):1716-9. doi: 10.1126/science.276.5319.1716.
5
Characterisation of a second protein encoded by the differentially regulated LmcDNA16 gene family of Leishmania major.硕大利什曼原虫差异调控的LmcDNA16基因家族所编码的第二种蛋白质的特性分析
Mol Biochem Parasitol. 1997 Apr;85(2):221-31. doi: 10.1016/s0166-6851(97)02829-6.
6
Histone H1 expression varies during the Leishmania major life cycle.组蛋白H1的表达在硕大利什曼原虫的生命周期中会发生变化。
Mol Biochem Parasitol. 1997 Feb;84(2):215-27. doi: 10.1016/s0166-6851(96)02801-0.
7
Unravelling the Leishmania genome.解析利什曼原虫基因组
Curr Opin Genet Dev. 1996 Dec;6(6):704-10. doi: 10.1016/s0959-437x(96)80024-4.
8
DNA vaccines against malaria: immunogenicity and protection in a rodent model.抗疟疾DNA疫苗：啮齿动物模型中的免疫原性与保护作用
J Pharm Sci. 1996 Dec;85(12):1294-300. doi: 10.1021/js960147h.
9
Leishmaniasis in Texas: epidemiology and clinical aspects of human cases.德克萨斯州的利什曼病：人类病例的流行病学和临床特征
Am J Trop Med Hyg. 1996 Nov;55(5):547-55. doi: 10.4269/ajtmh.1996.55.547.
10
An expressed sequence tag analysis of a full-length, spliced-leader cDNA library from Leishmania major promastigotes.对来自硕大利什曼原虫前鞭毛体的全长、剪接前导cDNA文库的表达序列标签分析。
Mol Biochem Parasitol. 1996 Feb-Mar;76(1-2):345-8. doi: 10.1016/0166-6851(95)02569-3.
Zotero 插件