Danilenko V N, Akopiants K E, Sizova I A, Michurina T A
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Genetika. 1997 Nov;33(11):1478-86.
The nucleotide sequence of a BglII-PstI DNA fragment that contains the cloned aphVIII gene from the Streptomyces rimosus P3 strain, the producer of oxytetracycline, was determined. It was established that the aph gene encodes neomycin phosphotransferase that differs by substrate specificity from neomycin phosphotransferases encoded by aph genes in producers of aminoglycoside antibiotics and clinical bacterial strains. The gene was shown to be 777 bp in length with the mean GC content equal to 67%. The amino acid sequence possesses all highly conserved regions typical for aminoglycoside phosphotransferases; however, this sequence contained several amino acid substitutions that have been detected for the first time, including those in the domain responsible for the association with antibiotics.
测定了包含来自土霉素产生菌龟裂链霉菌P3菌株克隆的aphVIII基因的BglII - PstI DNA片段的核苷酸序列。已确定aph基因编码新霉素磷酸转移酶，其底物特异性与氨基糖苷类抗生素产生菌和临床细菌菌株中aph基因编码的新霉素磷酸转移酶不同。该基因长度为777 bp，平均GC含量为67%。氨基酸序列具有氨基糖苷磷酸转移酶典型的所有高度保守区域；然而，该序列包含几个首次检测到的氨基酸取代，包括在负责与抗生素结合的结构域中的取代。
