Warden C H, Fisler J S
Rowe Genetics Program, Department of Pediatrics, University of California, Davis 95616, USA.
Methods. 1997 Dec;13(4):347-57. doi: 10.1006/meth.1997.0543.
Biological variations influence population variations of many common traits. Identification of the biological basis of many common diseases has been particularly difficult, but new reagents and analytical tools will greatly facilitate this process. The goal of this review is to discuss how to identify the biological basis of common traits by using mouse models. No single method will work for all traits. Understanding complex problems will require broadly based holistic approaches that use a wide array of tools and resources. A multiplicity of developed methods together provide the tools needed to identify the biological basis of any common trait. These tools, whole-genome linkage maps, maps of expressed genes, and statistical methods, deal with the complexities of multiple loci or correlated traits. This review provides some criteria for making choices about the likely productive approaches at each stage in the process of finding genes that influence common traits.
生物变异影响许多常见性状的群体变异。确定许多常见疾病的生物学基础一直特别困难，但新的试剂和分析工具将极大地推动这一进程。本综述的目的是讨论如何利用小鼠模型确定常见性状的生物学基础。没有一种单一的方法适用于所有性状。理解复杂问题需要采用广泛的整体方法，使用各种各样的工具和资源。多种已开发的方法共同提供了确定任何常见性状生物学基础所需的工具。这些工具，即全基因组连锁图谱、表达基因图谱和统计方法，应对多个基因座或相关性状的复杂性。本综述提供了一些标准，用于在寻找影响常见性状的基因的过程中，对每个阶段可能有效的方法做出选择。
