荧光素对鲁米诺 - H₂O₂ - 辣根过氧化物酶化学发光的增强作用：能量转移过程。
Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process.
作者信息
Navas Díaz A, González García J A, Lovillo J
机构信息
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Málaga, Spain.
出版信息
J Biolumin Chemilumin. 1997 Jul-Aug;12(4):199-205. doi: 10.1002/(SICI)1099-1271(199707/08)12:4<199::AID-BIO445>3.0.CO;2-U.
The chemiluminescence of the luminol-H2O2-horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than three times greater than without fluorescein; however, the emission duration was shorter. The emission spectra in the presence of fluorescein had two maxima (425 and 535 nm) and the enhancement was dependent on pH and fluorescein concentration. A mechanism is proposed to explain these effects.
荧光素可增强鲁米诺 - H₂O₂ - 辣根过氧化物酶体系的化学发光。荧光素通过从鲁米诺到荧光素的能量转移过程，使鲁米诺化学发光增强，其增强效果类似于酚酞。含有荧光素的鲁米诺的最大强度和总化学发光发射（380至580纳米之间）比不含荧光素时大三倍以上；然而，发射持续时间较短。存在荧光素时的发射光谱有两个最大值（425和535纳米），且增强作用取决于pH值和荧光素浓度。本文提出了一种机制来解释这些效应。
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