John A M, Davis G R, Sosulski F W
Arch Int Physiol Biochim. 1979 Dec;87(5):997-1004. doi: 10.3109/13813457909070548.
Larvae of Tenebrio molitor L;, Gembloux strain, race F, having an average initial weight of 10 mg were reared for 4 weeks at 27 +/- 0.25 degrees C and 65 +/- 5% relative humidity on diets containing an amino-acid mixture resembling the composition of larval tissues; Each of 14 amino acids was tested individually at levels of 0, 10, 20, 50, 100 and 200% of the amount found in larval tissues, while the concentration of other amino acids remained constant. Diets were maintained isonitrogenous at 1.6% nitrogen with supplementary glutamic acid, glycine, serine and tyrosine; Maximum fresh weight gains and dry weights were achieved by larvae fed reference levels of all essential amino acids except phenylalanine, threonine and tryptophan. Maximum growth was achieved by larvae fed 50% of the phenylalanine level found in larval tissues, Threonine and tryptophan were the limiting amino acids in this study and are probably required in the diet in excess of twice the concentration occuring in larval tissues, Probable ranges for quantitative amino-acid requirements of T. molitor were determined and suggestions were made for improving the nutritional adequacy of the amino-acid mixture.
黄粉虫（黄粉虫属，根特菌株，F族）幼虫，初始平均体重为10毫克，在27±0.25摄氏度、相对湿度65±5%的条件下，以含有类似于幼虫组织成分的氨基酸混合物的饲料饲养4周；14种氨基酸中的每一种都分别以幼虫组织中含量的0%、10%、20%、50%、100%和200%的水平进行测试，而其他氨基酸的浓度保持不变。饲料通过补充谷氨酸、甘氨酸、丝氨酸和酪氨酸保持氮含量为1.6%的等氮状态；除苯丙氨酸、苏氨酸和色氨酸外，喂食所有必需氨基酸参考水平的幼虫实现了最大鲜重增加和干重。喂食幼虫组织中苯丙氨酸水平50%的幼虫实现了最大生长。苏氨酸和色氨酸是本研究中的限制性氨基酸，可能在饲料中的需求量超过幼虫组织中浓度的两倍。确定了黄粉虫定量氨基酸需求的可能范围，并就提高氨基酸混合物的营养充足性提出了建议。
