Markopoulos A K, Belazi M A, Drakoulakos D
Aristole University of Thessaloniki, School of Dentistry, Department of Oral Medicine/Pathology, Greece.
Diabetes Res Clin Pract. 1997 Dec;38(3):169-72. doi: 10.1016/s0168-8227(97)00078-8.
The aim of this study was to investigate the presence of glutamic acid decarboxylase (GAD) autoantibodies in the saliva of children with type 1 diabetes. A total of 30 diabetic children were used in this study while 80 healthy individuals of similar age were used as controls. Total saliva and peripheral blood was collected from all diabetic children and controls. Determination of GAD autoantibodies was performed using an enzyme immunoassay (Varelisa) which is based on a recombinant human GAD65. The results of this study showed that GAD autoantibodies were found to be present in 21 out of 30 diabetic serum samples, while they were present in all samples of diabetic saliva. In contrast, they were absent from serum and saliva of healthy controls. Since GAD is considered one of the basic antigenic targets of the autoimmune process in type 1 diabetes in the pancreatic beta cells, its presence in the saliva may be relevant as a predictive marker.
本研究的目的是调查1型糖尿病儿童唾液中谷氨酸脱羧酶（GAD）自身抗体的存在情况。本研究共纳入30名糖尿病儿童，同时选取80名年龄相仿的健康个体作为对照。收集了所有糖尿病儿童和对照的全唾液和外周血。使用基于重组人GAD65的酶免疫测定法（Varelisa）测定GAD自身抗体。本研究结果显示，30份糖尿病血清样本中有21份检测到GAD自身抗体，而所有糖尿病唾液样本中均检测到该抗体。相比之下，健康对照的血清和唾液中均未检测到。由于GAD被认为是1型糖尿病胰腺β细胞自身免疫过程的基本抗原靶点之一，其在唾液中的存在可能作为一种预测标志物具有重要意义。
