Garcia-Godoy F, Summitt J B, Donly K J
Department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio 78284-7888, USA.
J Clin Pediatr Dent. 1997 Winter;21(2):141-3.
This study evaluated the artificial caries resistance provided to white spot lesions by sealing with an unfilled resin. Twenty extracted molar teeth were coated with an acid-resistant varnish (ARV), except for two 5 mm x 2 mm enamel windows (mesial and distal), and immersed for 5 weeks in an acidified gel brought to a pH of 4.2 with lactic acid. White spot lesions (not frank caries) were created in each window. The teeth were removed from the gel, rinsed and dried. One window was divided into two equal parts; one half was covered with the ARV (Group 1); the other half was not covered (Group 2). The other window was treated with 37% phosphoric acid for 20 sec, rinsed with water for 20 sec, dried and sealed with Prisma Universal Bond 2 Adhesive (Group 3). The teeth were reimmersed in the acidified gel for 40 days until frank caries was induced in Group 2. Specimens were then removed from the gel, rinsed, and sectioned occlusogingivally. Sections were polished to approximately 100 microns, imbibed in water, and analyzed using polarized light microscopy. Depth of demineralization was evaluated in the varnished, sealed and frankly carious areas. The mean (SD) lesion depths were as follows: Group 1 (under ARV) 366 microns (103 microns); Group 2 (frank caries) 746 microns (219 microns); Group 3 (under sealed area) 298 microns (111 microns). ANOVA and Student-Newman-Keuls procedure were used to compare group means. The mean depths of demineralization under the ARV (Group 1) and the unfilled resin sealant (Group 2) were significantly less than for the unprotected enamel (Group 2) (p > 0.0001). Results indicate the potential effectiveness of sealing white spot lesions with an unfilled resin to prevent further demineralization.
本研究评估了用未填充树脂封闭对白斑病变的人工抗龋能力。除了两个5毫米×2毫米的釉质窗口（近中和远中）外，对20颗拔除的磨牙涂覆耐酸清漆（ARV），并将其浸入用乳酸调至pH值为4.2的酸化凝胶中5周。在每个窗口中形成白斑病变（非明显龋损）。将牙齿从凝胶中取出，冲洗并干燥。一个窗口分为两个相等的部分；一半用ARV覆盖（第1组）；另一半不覆盖（第2组）。另一个窗口用37%的磷酸处理20秒，用水冲洗20秒，干燥并用Prisma Universal Bond 2粘合剂封闭（第3组）。将牙齿重新浸入酸化凝胶中40天，直到第2组出现明显龋损。然后将标本从凝胶中取出，冲洗，并沿咬合龈向切片。切片抛光至约100微米，吸水，并使用偏光显微镜进行分析。评估涂漆、封闭和明显龋损区域的脱矿深度。平均（标准差）病变深度如下：第1组（在ARV下）366微米（103微米）；第2组（明显龋损）746微米（219微米）；第3组（在封闭区域下）298微米（111微米）。使用方差分析和Student-Newman-Keuls程序比较组均值。ARV（第1组）和未填充树脂封闭剂（第3组）下的平均脱矿深度明显小于未保护釉质（第2组）（p>0.0001）。结果表明用未填充树脂封闭白斑病变以防止进一步脱矿的潜在有效性。
