Chaffanet M, Popovici C, Leroux D, Jacrot M, Adélaïde J, Dastugue N, Grégoire M J, Hagemeijer A, Lafage-Pochitaloff M, Birnbaum D, Pébusque M J
Laboratoire d'Oncologie Moléculaire, U.119 INSERM, Institut de Cancérologie et d'Immunologie de Marseille, France.
Oncogene. 1998 Feb 19;16(7):945-9. doi: 10.1038/sj.onc.1201601.
A stem-cell myeloproliferative disorder involving T- and B-cell, and myeloid lineages, is associated with three different translocations with a breakpoint in region p11-12 of chromosome 8: t(6;8)(q27;p11), t(8;9)(p11;q33), and t(8;13)(p12;q12), respectively. Using fluorescence in situ hybridization (FISH), we have analysed blood cells from a series of five patients carrying these different translocations. We have identified cosmids from chromosome region 8p11-12 that span the breakpoint in all the cases. They are specific for the FCFR1 gene that encodes a receptor for members of the FGF family. The breakpoint was further detected by Southern and pulsed-field gel electrophoresis analyses with probes from the FGFR1 locus.
一种涉及T细胞、B细胞和髓系谱系的干细胞骨髓增殖性疾病，与三种不同的易位相关，其断点分别位于8号染色体的p11 - 12区域：分别为t(6;8)(q27;p11)、t(8;9)(p11;q33)和t(8;13)(p12;q12)。我们使用荧光原位杂交（FISH）技术，分析了一系列携带这些不同易位的五名患者的血细胞。我们已鉴定出8号染色体区域8p11 - 12的黏粒，这些黏粒在所有病例中均跨越断点。它们对编码FGF家族成员受体的FCFR1基因具有特异性。通过使用来自FGFR1基因座的探针进行Southern杂交和脉冲场凝胶电泳分析，进一步检测到了断点。
