Pax5基因在人类造血细胞和组织中的表达：与免疫缺陷供体的比较。
Expression of Pax5 gene in human haematopoietic cells and tissues: comparison with immunodeficient donors.
作者信息
Kaneko H, Ariyasu T, Inoue R, Fukao T, Kasahara K, Teramoto T, Matsui E, Hayakawa S, Kondo N
机构信息
Department of Paediatrics, Gifu University School of Medicine, Japan.
出版信息
Clin Exp Immunol. 1998 Feb;111(2):339-44. doi: 10.1046/j.1365-2249.1998.00509.x.
In mice, Pax5 gene is indispensable for B cell development. Pax5-deficient mice fail to produce mature B cells owing to complete arrest of B cell development at a precursor stage. However, the lineage and stage of human Pax5 gene expression have remained elusive. In this investigation expression of the human Pax5 gene was studied. Pax5 gene expression was detected in B cell lines but not in myeloma cell lines. CD19 expression was correlated with Pax5 gene expression. Adult spleen and bone marrow and fetal spleen and liver showed strong Pax5 gene expression, as did the corresponding mouse tissues, as reported previously. In common variable immunodeficiency (CVID) peripheral blood lymphocytes (PBL) with a decreased number of B cells, no Pax5 gene expression was detected. Some CVID PBL stimulated with IL-2, IL-10 and anti-CD40 monoclonal antibody, expressed the Pax5 gene. Defect of Pax5 gene expression in CVID may be caused by regulatory T cell disorder.
在小鼠中，Pax5基因对于B细胞发育不可或缺。Pax5基因缺陷型小鼠无法产生成熟B细胞，因为B细胞发育在前体阶段完全停滞。然而，人类Pax5基因表达的谱系和阶段仍不清楚。在本研究中，对人类Pax5基因的表达进行了研究。在B细胞系中检测到Pax5基因表达，但在骨髓瘤细胞系中未检测到。CD19表达与Pax5基因表达相关。如先前报道，成人脾脏和骨髓以及胎儿脾脏和肝脏显示出强烈的Pax5基因表达，相应的小鼠组织也是如此。在B细胞数量减少的常见可变免疫缺陷（CVID）外周血淋巴细胞（PBL）中，未检测到Pax5基因表达。一些用白细胞介素-2、白细胞介素-10和抗CD40单克隆抗体刺激的CVID PBL表达了Pax5基因。CVID中Pax5基因表达缺陷可能由调节性T细胞紊乱引起。
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