Rols M P, Delteil C, Golzio M, Dumond P, Cros S, Teissie J
Institute of Pharmacology and Structural Biology, UPR 9062, Toulouse, France.
Nat Biotechnol. 1998 Feb;16(2):168-71. doi: 10.1038/nbt0298-168.
We show that efficient permeabilization of murine melanoma can be obtained in vivo by applying electric pulses. More than 80% of the cell population is affected as shown by the penetration of propidium iodide. A protein, beta-galactosidase, can be transferred and expressed into the cells by incorporating either the protein or a plasmid carrying the reporter gene with respective efficiencies of 20% and 4%. This is obtained by a direct injection of either the protein or the plasmid in the tumor, followed by the application of electric pulses with surface electrodes in contact with the skin. This approach is simple and safe to use, reproducible, and specific; moreover, it is potentially applicable to a wide variety of tissues, cell types, and animals.
我们表明，通过施加电脉冲可在体内实现对小鼠黑色素瘤的高效通透化。如碘化丙啶的穿透所示，超过80%的细胞群体受到影响。通过将蛋白质或携带报告基因的质粒导入细胞，可将一种蛋白质β-半乳糖苷酶转移并表达，其各自的效率分别为20%和4%。这是通过在肿瘤中直接注射蛋白质或质粒，随后使用与皮肤接触的表面电极施加电脉冲来实现的。这种方法使用简单、安全、可重复且具有特异性；此外，它有可能适用于多种组织、细胞类型和动物。
