Panzacchi G, Demarchi B, Busca G, Protasoni G, Golin R, Stella A
Istituto di Clinica Medica e Terapia Medica, University of Milan, Ospedale Maggiore, Italy.
J Hypertens. 1997 Dec;15(12 Pt 2):1785-9. doi: 10.1097/00004872-199715120-00090.
To investigate the effects of the interaction between adenosine receptors and renal nerves on urinary sodium excretion and glomerular filtration rate.
The effects on water and sodium excretion and glomerular filtration rate of A1 [2-chloro-N6-cyclopentyl-adenosine (CCPA)] and A2 [2-hesinyl-5'-N-ethyl-carboxamido-adenosine (2HE-NECA)] adenosine agonists were studied in anaesthetized rats with one kidney surgically denervated. Arterial blood pressure, heart rate and rate of urine flow from each kidney were continuously recorded; inulin clearance was used as an index of glomerular filtration rate. The experiments were performed with three groups of rats, into which, after a control period of 20 min, CCPA, 2HE-NECA or vehicle was infused for two subsequent 20 min periods.
During infusion of CCPA, the slight decrease in arterial pressure was associated with a transient decrease in glomerular filtration rate and marked long-lasting decreases in heart rate, water and sodium excretion and fractional sodium excretion. The response of the innervated kidney was similar to the response of the denervated kidney. Infusion of 2HE-NECA caused decreases in arterial pressure, glomerular filtration rate and excretion of water and sodium associated with an increase in heart rate. The reduction of water and sodium excretion from the innervated kidney was larger than that from the denervated kidney.
Activation both of A1 and of A2 receptor causes a reduction in urinary water and sodium excretion. The renal response to activation of A2 receptors is enhanced by the presence of renal nerves, whereas the response to activation of A1 receptors is not influenced by renal nerves.
研究腺苷受体与肾神经之间的相互作用对尿钠排泄和肾小球滤过率的影响。
在一侧肾脏手术去神经支配的麻醉大鼠中，研究A1[2-氯-N6-环戊基腺苷（CCPA）]和A2[2-己酰基-5'-N-乙基-羧酰胺基腺苷（2HE-NECA）]腺苷激动剂对水和钠排泄以及肾小球滤过率的影响。连续记录动脉血压、心率和每个肾脏的尿流率；菊粉清除率用作肾小球滤过率的指标。实验用三组大鼠进行，在20分钟的对照期后，随后的两个20分钟时间段分别输注CCPA、2HE-NECA或赋形剂。
在输注CCPA期间，动脉压的轻微下降与肾小球滤过率的短暂下降以及心率、水和钠排泄及钠排泄分数的显著持久下降有关。去神经支配肾脏的反应与未去神经支配肾脏的反应相似。输注2HE-NECA导致动脉压、肾小球滤过率以及水和钠排泄减少，同时心率增加。去神经支配肾脏的水和钠排泄减少幅度大于未去神经支配的肾脏。
A1和A2受体的激活均导致尿水和钠排泄减少。肾神经的存在增强了肾脏对A2受体激活的反应，而对A1受体激活的反应不受肾神经影响。
