Saldaña A, Harris R A, Orn A, Sousa O E
Center for Research and Diagnosis of Parasitic Diseases, Faculty of Medicine, University of Panama.
J Parasitol. 1998 Feb;84(1):67-73.
Trypanosoma rangeli infects humans nonpathologically in some areas of Central and South America. Due to morphological and antigenic similarities with T. cruzi, the clear identification of this parasite is an important task. Here, we describe the identification and purification of a specific 48-kDa antigen from T. rangeli. By western blotting analysis, this molecule was not detected in T. cruzi epimastigotes and in Leishmania sp. promastigotes. Fluorescence-activated cell sorter analysis demonstrated that the protein is expressed uniformly by the T. rangeli cell population during axenic culture. Additionally, following immunostaining, a particular subcellular localization (present in organelles) is proposed for this antigen. These results suggest that this 48-kDa antigen may be a useful marker for the identification and characterization of T. rangeli isolates.
在中美洲和南美洲的一些地区，兰氏锥虫可非致病性地感染人类。由于其在形态和抗原方面与克氏锥虫相似，因此明确鉴定这种寄生虫是一项重要任务。在此，我们描述了从兰氏锥虫中鉴定和纯化一种特定的48 kDa抗原的过程。通过蛋白质印迹分析，在克氏锥虫前鞭毛体和利什曼原虫前鞭毛体中未检测到该分子。荧光激活细胞分选分析表明，在无细胞培养期间，兰氏锥虫细胞群体均匀表达该蛋白。此外，免疫染色后，推测该抗原存在于特定的亚细胞定位（细胞器中）。这些结果表明，这种48 kDa抗原可能是鉴定和表征兰氏锥虫分离株的有用标志物。
