Kadhim M A, Marsden S J, Wright E G
Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire, UK.
Int J Radiat Biol. 1998 Feb;73(2):143-8. doi: 10.1080/095530098142518.
To determine whether chromosomal instability is induced in human diploid fibroblasts by ionizing radiation and to investigate the effects of radiation quality by comparing X-rays, neutrons and alpha-particles.
Cells from two human diploid fibroblast lines, HF12 and HF19, were irradiated and analysed cytogenetically at 3, 20 and 35 population doublings post-irradiation.
Exposure of HF19 cells to neutrons and alpha-particles resulted in a consistently increased frequency of unstable aberrations, particularly chromatid-type aberrations, compared to control cultures. Aberration frequency after X-irradiation was not significantly greater than controls at 20 population doublings but was significantly increased after 35 population doublings, although not to the same level as that following neutron or alpha-irradiation. No chromosomal instability was demonstrated in the progeny of HF12 cells after X-, neutron or alpha-particle irradiation.
The data are consistent with the progeny of irradiated HF19 cells expressing chromosomal instability. All three radiations are effective in inducing instability, but the expression of the phenotype is influenced by radiation quality. The absence of radiation-induced chromosomal instability in HF12 cells may reflect the influence of genetic factors.
确定电离辐射是否会在人二倍体成纤维细胞中诱发染色体不稳定，并通过比较X射线、中子和α粒子来研究辐射质量的影响。
对来自两个人二倍体成纤维细胞系HF12和HF19的细胞进行辐照，并在辐照后第3、20和35次群体倍增时进行细胞遗传学分析。
与对照培养物相比，HF19细胞暴露于中子和α粒子后，不稳定畸变的频率持续增加，尤其是染色单体型畸变。X射线辐照后，在第20次群体倍增时畸变频率与对照相比无显著增加，但在第35次群体倍增后显著增加，尽管未达到中子或α粒子辐照后的水平。HF12细胞在接受X射线、中子或α粒子辐照后，其后代未表现出染色体不稳定。
数据表明辐照后的HF19细胞后代表现出染色体不稳定。所有三种辐射均能有效诱发不稳定，但表型的表达受辐射质量影响。HF12细胞中未出现辐射诱导的染色体不稳定可能反映了遗传因素的影响。
