Beaulieu M J, Li H, Bergeron J, Ross G, Auger F A, Rouabhia M
Laboratoire de Recherche des Grandes Brûlés/LOEX, Surgery Department, Laval University, Quebec, Canada.
Cell Transplant. 1998 Jan-Feb;7(1):11-23. doi: 10.1177/096368979800700104.
This study describes the involvement of male-specific minor histocompatibility antigen H-Y in vitro cultured epidermal equivalent (EE) rejection. Male and female Balb/c or C3H/HeN keratinocytes were isolated and cultured separately. Male EE were grafted onto adult male (isografts) and adult female (H-Y allografts) mice. As controls, Balb/c EE were grafted onto adult C3H/HeN (complete allografts) mice. Fourteen, 21, and 30 days postgrafting, histological studies showed well-organized cutaneous tissues with complete basement membranes (laminin and type IV collagen deposition) in H-Y allografts compared to the isografts. This cutaneous organization was altered 150 days postgrafting, which is a sign of the H-Y EE allograft rejection. Complete allografts were totally rejected 21 days postgrafting. Immunological studies revealed leucocyte infiltration of H-Y allografts. Significant infiltration was detected even 150 days postgrafting. Leucocyte phenotyping revealed the presence of Mac-I+, CD8+ and CD4+ cells in the H-Y allografts. Humoral immune analysis revealed the presence of circulating anti-H-Y allogeneic keratinocyte cytotoxic antibodies in female recipient sera. Our data suggest that male-specific minor histocompatibility antigen H-Y induces cellular and humoral activation of the recipient immune system even after grafting EE free of cutaneous active immune cells such as T lymphocytes and Langerhans cells.
本研究描述了雄性特异性次要组织相容性抗原H-Y在体外培养的表皮替代物（EE）排斥反应中的作用。分别分离培养雄性和雌性Balb/c或C3H/HeN角质形成细胞。将雄性EE移植到成年雄性（同基因移植）和成年雌性（H-Y异基因移植）小鼠身上。作为对照，将Balb/c EE移植到成年C3H/HeN（完全异基因移植）小鼠身上。移植后14天、21天和30天，组织学研究显示，与同基因移植相比，H-Y异基因移植中有组织良好的皮肤组织，具有完整的基底膜（层粘连蛋白和IV型胶原沉积）。移植后150天，这种皮肤组织发生改变，这是H-Y EE异基因移植排斥的迹象。完全异基因移植在移植后21天被完全排斥。免疫学研究显示H-Y异基因移植中有白细胞浸润。甚至在移植后150天也检测到明显的浸润。白细胞表型分析显示H-Y异基因移植中存在Mac-I+、CD8+和CD4+细胞。体液免疫分析显示雌性受体血清中存在循环抗H-Y异基因角质形成细胞细胞毒性抗体。我们的数据表明，即使在移植不含皮肤活性免疫细胞（如T淋巴细胞和朗格汉斯细胞）的EE后，雄性特异性次要组织相容性抗原H-Y也会诱导受体免疫系统的细胞和体液激活。
