类风湿性滑膜细胞培养的多细胞球体对人软骨的侵袭——一种用于类风湿性关节炎的新型体外模型系统
Invasion of human cartilage by cultured multicellular spheroids of rheumatoid synovial cells--a novel in vitro model system for rheumatoid arthritis.
作者信息
Ermis A, Müller B, Hopf T, Hopf C, Remberger K, Jüsten H P, Welter C, Hanselmann R
机构信息
Institute of Human Genetics, Department of Orthopedics, University of Homburg, Saarland, Germany.
出版信息
J Rheumatol. 1998 Feb;25(2):208-13.
OBJECTIVE
A new 3-dimensional cell culture system was established to examine the invasion of rheumatoid synovial cells into cartilage.
METHODS
Synovial cells from 20 patients with rheumatoid arthritis (RA) and 15 patients with osteoarthritis (OA) were co-cultured as multicellular spheroids with cartilage fragments.
RESULTS
After 4 weeks the rheumatoid spheroids eroded the cartilage. The destruction of cartilage was supported by a high expression level of cathepsin D and matrix metalloproteinases. In contrast, the OA tissue showed attachment to the cartilage only.
CONCLUSION
Our results indicate that spheroid/cartilage co-cultures seem to be a suitable in vitro model for the study of destructive cellular mechanisms that occur in RA.
目的
建立一种新的三维细胞培养系统，以研究类风湿性滑膜细胞对软骨的侵袭。
方法
将20例类风湿性关节炎（RA）患者和15例骨关节炎（OA）患者的滑膜细胞与软骨碎片共同培养成多细胞球体。
结果
4周后，类风湿性球体侵蚀了软骨。组织蛋白酶D和基质金属蛋白酶的高表达水平支持了软骨的破坏。相比之下，OA组织仅显示与软骨附着。
结论
我们的结果表明，球体/软骨共培养似乎是研究RA中发生的破坏性细胞机制的合适体外模型。
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