Yamanishi Y, Hiyama K, Maeda H, Ishioka S, Murakami T, Hiyama E, Kurose Y, Shay J W, Yamakido M
Second Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
J Rheumatol. 1998 Feb;25(2):214-20.
To determine whether the presence of telomerase activity in synovial tissues could provide insights into the pathogenesis of rheumatoid arthritis (RA).
Synovial tissue samples obtained from patients with RA or osteoarthritis (OA) were examined for telomerase activity using the telomeric repeat amplification protocol (TRAP).
Telomerase activity was detected in over half the RA synovial tissues (14/25, 56%) but in no OA synovial tissue samples (0/15). Telomerase activity was detected in the mononuclear cells isolated from telomerase-positive RA synovial tissue, but not in cultured adherent cells. In RA synovial tissue with positive telomerase activity mononuclear cell infiltration levels were increased (p < 0.001). In addition, patients with RA with positive telomerase activity had undergone joint operations at an earlier age (p = 0.030) and more often (p = 0.023) compared to those without telomerase activity.
Telomerase activity in RA synovial tissue is likely derived from infiltrating mononuclear cells and may be involved in the pathogenesis of RA. Clinically, the presence of telomerase activity in RA may be an indicator of a more aggressive phenotype.
确定滑膜组织中端粒酶活性的存在是否能为类风湿关节炎（RA）的发病机制提供见解。
使用端粒重复序列扩增法（TRAP）检测从RA患者或骨关节炎（OA）患者获取的滑膜组织样本中的端粒酶活性。
超过半数的RA滑膜组织（14/25，56%）检测到端粒酶活性，而OA滑膜组织样本中均未检测到（0/15）。从端粒酶阳性的RA滑膜组织中分离出的单核细胞检测到端粒酶活性，但培养的贴壁细胞中未检测到。在端粒酶活性阳性的RA滑膜组织中，单核细胞浸润水平升高（p < 0.001）。此外，与无端粒酶活性的RA患者相比，端粒酶活性阳性的RA患者在更早的年龄接受关节手术（p = 0.030），且手术频率更高（p = 0.023）。
RA滑膜组织中的端粒酶活性可能来源于浸润的单核细胞，并且可能参与RA的发病机制。临床上，RA中端粒酶活性的存在可能是更具侵袭性表型的一个指标。
