Agapitos E, Nanas J, Kavantzas N, Nanas S, Yova D, Trikoupis A, Davaris P
Department of Pathology, Medical School University of Athens, Greece.
Gen Diagn Pathol. 1997 Dec;143(4):215-8.
Thrombolytic therapy improves left ventricular ejection fraction and survival. Using the image analysis method, the study was undertaken to evaluate the effects of intraaortic balloon pump used in conjunction with reperfusion in reducing infarct size by measuring the infarct area. Also, the usefulness of image analysis as an objective method for the measurement of myocardial infarct area is evaluated.
22 dogs were subjected to proximal left anterior descending coronary artery occlusion. The dogs were classified into 3 groups. In group I (n = 7), occlusion lasted for 6 hours. In group II (n = 6), 2 hours of occlusion were followed by reperfusion. In group III, (n = 9) after 2 hours of occlusion, the dogs were assisted with the intraaortic balloon pump throughout the 4 hours of reperfusion. The measurement of the infarcted area components (fragmentation, edema, hemorrhage and polymorphonuclear infiltrations) was followed by a semiautomatic method including in connection a microscope photographic camera, a scanner and a computer with the appropriate software. The results were analyzed statistically using the t-test.
In group I, the mean value of fragmentation was 28.2%, in group II, 10.1% (p < 0.01 versus group I) and in group III, 3.9% (p < 0.01 versus I and p < 0.05 versus group II).
Reperfusion and intraaortic balloon pump increased the salvage of the ischemic myocardium over that achieved by reperfusion alone in a canine occlusion - reperfusion model. Image analysis could be considered as an accurate and objective method for the measurement of the myocardial infarct area in the experimental model of our study and it could be used in any other experimental study in which the accurate measurement of myocardial infarct area is needed.
溶栓治疗可改善左心室射血分数并提高生存率。本研究采用图像分析方法，通过测量梗死面积来评估主动脉内球囊反搏联合再灌注在减少梗死面积方面的效果。此外，还评估了图像分析作为测量心肌梗死面积的客观方法的实用性。
对22只犬进行左冠状动脉前降支近端闭塞。将犬分为3组。第一组（n = 7），闭塞持续6小时。第二组（n = 6），闭塞2小时后进行再灌注。第三组（n = 9），闭塞2小时后，在整个4小时的再灌注过程中使用主动脉内球囊反搏辅助。采用半自动方法测量梗死区域成分（碎裂、水肿、出血和多形核浸润），该方法包括连接显微镜摄影相机、扫描仪和装有适当软件的计算机。使用t检验对结果进行统计学分析。
第一组中，碎裂的平均值为28.2%，第二组为10.1%（与第一组相比，p < 0.01），第三组为3.9%（与第一组相比，p < 0.01；与第二组相比，p < 0.05）。
在犬闭塞-再灌注模型中，再灌注和主动脉内球囊反搏比单纯再灌注能增加缺血心肌的挽救。在本研究的实验模型中，图像分析可被视为测量心肌梗死面积的准确且客观的方法，并且可用于任何其他需要准确测量心肌梗死面积的实验研究。
