Bystron' I P, Otellin V A, Wierzba-Bobrowicz T, Dymecki J
Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg.
Tsitologiia. 1997;39(7):560-5.
Dynamics of survival, differentiation and migration of human fetal cells after their transplantation into the rat brain without immunosuppression was studied using routine histology, electron microscopy and immunocytochemistry. A cell suspension prepared from the ventral mesencephalon of human embryos 7-8 weeks of gestation was injected into the striatum of rats-recipients. The graft development depends on the intensity of immune reaction. Under a weak reaction, the viability and differentiation of human embryo cells in the rat brain were observed within the three months of the experiment. The grafted cells conserve their mediator specificity, some of them being seen to migrate into the host (rat) tissue beyond the graft. In the transplant neuropil various types of cell contacts were observed, including synapses. The described method makes its possible to study the human nervous tissue histogenesis in an abnormal environment.
在不进行免疫抑制的情况下，将人胎儿细胞移植到大鼠脑内后，运用常规组织学、电子显微镜和免疫细胞化学方法，对其存活、分化和迁移的动态过程进行了研究。从妊娠7 - 8周的人胚胎腹侧中脑制备的细胞悬液，被注入到受体大鼠的纹状体中。移植物的发育取决于免疫反应的强度。在弱反应情况下，在实验的三个月内观察到了人胚胎细胞在大鼠脑内的存活和分化。移植的细胞保留了它们的介质特异性，其中一些细胞迁移到移植部位以外的宿主（大鼠）组织中。在移植的神经毡中观察到了各种类型的细胞接触，包括突触。所描述的方法使得在异常环境中研究人类神经组织的组织发生成为可能。
