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本文引用的文献

1
The calmodulin-stimulated ATPase of maize coleoptiles is a 140000-Mr polypeptide.钙调素刺激的玉米幼茎 ATP 酶是一种 140000Mr 的多肽。
Planta. 1988 Nov;176(2):283-5. doi: 10.1007/BF00392457.
2
Plasma Membrane Ca-ATPase of Radish Seedlings : II. Regulation by Calmodulin.萝卜幼苗质膜 Ca-ATP 酶：Ⅱ.钙调蛋白的调节。
Plant Physiol. 1992 Mar;98(3):1202-6. doi: 10.1104/pp.98.3.1202.
3
Plasma Membrane Ca-ATPase of Radish Seedlings : I. Biochemical Characterization Using ITP as a Substrate.萝卜幼苗质膜 Ca-ATP 酶的研究：I. 以三磷酸肌醇为底物的生化特性。
Plant Physiol. 1992 Mar;98(3):1196-201. doi: 10.1104/pp.98.3.1196.
4
Calcium-pumping ATPases in vesicles from carrot cells : stimulation by calmodulin or phosphatidylserine, and formation of a 120 kilodalton phosphoenzyme.胡萝卜细胞囊泡中的钙泵ATP酶：受钙调蛋白或磷脂酰丝氨酸刺激及120千道尔顿磷酸酶的形成
Plant Physiol. 1991 Dec;97(4):1535-44. doi: 10.1104/pp.97.4.1535.
5
Solubilization and reconstitution of ca pump from corn leaf plasma membrane.玉米叶片质膜上钙泵的增溶与重组
Plant Physiol. 1991 Jun;96(2):565-70. doi: 10.1104/pp.96.2.565.
6
Functional Reconstitution of an ATP-Driven Ca-Transport System from the Plasma Membrane of Commelina communis L.鸭跖草质膜ATP驱动钙转运系统的功能重建
Plant Physiol. 1990 Oct;94(2):634-40. doi: 10.1104/pp.94.2.634.
7
Ca-translocating ATPase of the plant plasma membrane.植物质膜 Ca 转运 ATP 酶。
Plant Physiol. 1990 Oct;94(2):397-400. doi: 10.1104/pp.94.2.397.
8
Further Characterization of the Red Beet Plasma Membrane Ca-ATPase Using GTP as an Alternative Substrate.以GTP作为替代底物对红甜菜质膜Ca-ATP酶进行进一步表征。
Plant Physiol. 1990 Mar;92(3):747-54. doi: 10.1104/pp.92.3.747.
9
Identification and Characterization of the Ca-ATPase which Drives Active Transport of Ca at the Plasma Membrane of Radish Seedlings.鉴定并描述驱动萝卜幼苗质膜上钙离子主动运输的 Ca-ATP 酶。
Plant Physiol. 1989 Aug;90(4):1429-34. doi: 10.1104/pp.90.4.1429.
10
Calcium Transport in Sealed Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue : I. Characterization of a Ca-Pumping ATPase Associated with the Endoplasmic Reticulum.密封液泡中钙运输：一. 与内质网相关的 Ca2+-ATP 酶的特性。
Plant Physiol. 1987 Dec;85(4):1129-36. doi: 10.1104/pp.85.4.1129.

通过钙调蛋白 - 琼脂糖亲和层析法从萝卜幼苗中纯化质膜钙ATP酶

Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography.

作者信息

Bonza C, Carnelli A, Rasi-Caldogno F

机构信息

Dipartimento di Biologia L. Gorini, Università di Milano, via G. Celoria 26, 20133 Milano, Italy (C.B., A.C., F.R.-C.)

出版信息

Plant Physiol. 1998 Feb 1;116(2):845-51. doi: 10.1104/pp.116.2.845.

DOI:10.1104/pp.116.2.845
PMID:9490776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC35144/
Abstract

The Ca2+-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. PM purified by aqueous two-phase partitioning was solubilized with n-dodecyl beta-d-maltoside and applied to a CaM-agarose matrix. After various washings with decreasing Ca2+ concentrations, the Ca2+-ATPase was eluted with 5 mm ethylenediaminetetraacetate (EDTA). The EDTA-eluted fraction contained about 25% of the loaded Ca2+-ATPase activity, with a specific activity 70-fold higher than that of the starting PM fraction. The EDTA-eluted fraction was highly enriched in a 133-kD polypeptide, which was identified as the PM Ca2+-ATPase by 125I-CaM overlay and fluorescein-isothiocyanate labeling. The PM Ca2+-ATPase cross-reacted with an antiserum against a putative Ca2+-ATPase of the Arabidopsis thaliana chloroplast envelope.

摘要

采用批量法，通过钙调蛋白（CaM）亲和层析对萌发萝卜（Raphanus sativus L.）种子质膜（PM）的Ca2 + -ATP酶进行了纯化。用正十二烷基β-D-麦芽糖苷溶解经双水相分配纯化的质膜，并将其应用于CaM-琼脂糖基质。在用浓度逐渐降低的Ca2 +进行各种洗涤后，用5 mM乙二胺四乙酸（EDTA）洗脱Ca2 + -ATP酶。经EDTA洗脱的部分含有约25%的加载Ca2 + -ATP酶活性，比起始质膜部分的比活性高70倍。经EDTA洗脱的部分高度富集一种133-kD多肽，通过125I-CaM覆盖和异硫氰酸荧光素标记鉴定为质膜Ca2 + -ATP酶。质膜Ca2 + -ATP酶与抗拟南芥叶绿体被膜假定Ca2 + -ATP酶的抗血清发生交叉反应。