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2
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Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification.通过多重连接依赖探针扩增对40个核酸序列进行相对定量分析。
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Fluorescence-based directed termination PCR: direct mutation characterization without sequencing.基于荧光的定向终止PCR：无需测序的直接突变特征分析
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Mutational spectrum analysis of RNase H(35) deficient Saccharomyces cerevisiae using fluorescence-based directed termination PCR.使用基于荧光的定向终止PCR对核糖核酸酶H(35)缺陷型酿酒酵母进行突变谱分析。
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本文引用的文献

1
Slowly but surely towards better scanning for mutations.朝着更好地检测突变稳步迈进。
Trends Genet. 1997 Feb;13(2):43-6. doi: 10.1016/s0168-9525(97)01011-1.
2
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.使用嗜热栖热菌DNA聚合酶进行DNA测序以及对聚合酶链反应扩增的DNA进行直接测序。
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40. doi: 10.1073/pnas.85.24.9436.
3
PCR-SSCP: a simple and sensitive method for detection of mutations in the genomic DNA.聚合酶链反应-单链构象多态性分析：一种用于检测基因组DNA突变的简单且灵敏的方法。
PCR Methods Appl. 1991 Aug;1(1):34-8. doi: 10.1101/gr.1.1.34.

定向终止PCR：一种用于突变检测的一步法

Directed termination PCR: a one-step approach to mutation detection.

作者信息

Chen J, Hebert P D

机构信息

Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

出版信息

Nucleic Acids Res. 1998 Mar 15;26(6):1546-7. doi: 10.1093/nar/26.6.1546.

DOI:10.1093/nar/26.6.1546
PMID:9490805
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147424/
Abstract

We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for the detection of both insertions/deletions and single base pair substitutions in sequences up to 1 kb in length.

摘要

我们描述了一种基于聚合酶链式反应（PCR）的新方法，该方法通过将选择性DNA扩增和定向链终止整合到单个PCR反应中，从而生成嵌套终止片段。这些终止片段可以在变性或非变性聚丙烯酰胺凝胶中检测序列变异。该方法为检测长度达1 kb的序列中的插入/缺失和单碱基对替换提供了一种一步到位且高效的方法。