Characterization of 5-HT-sensitive potassium conductances in neonatal rat facial motoneurones in vitro.

1. The properties of the 5-HT-sensitive K+ conductance of neonatal rat facial motoneurones were examined in brainstem slices using whole-cell patch-clamp techniques. 2. In a small proportion of motoneurones, 5-hydroxytryptamine (5-HT) evoked an inward current mediated solely by a decrease in K+ conductance. The reversal potential (V5-HT) was dependent on the external K+ concentration and the 5-HT-evoked current (I5-HT) displayed a linear current-voltage (I-V) relationship. 3. In the remaining motoneurones, the 5-HT-evoked decrease in K+ conductance could only be observed in isolation once a concomitant 5-HT-mediated enhancement of the hyperpolarization-activated current, Ih, had been abolished with the Ih blocker, ZD-7288. 4. External Cs+ also abolished the Ih-mediated component of I5-HT but, in addition, blocked part of the 5-HT-sensitive K+ current. At potentials hyperpolarized to V5-HT, Cs+ voltage dependently blocked I5-HT while at potentials depolarized to V5-HT, I5-HT was largely unaffected. Ba2+ and Rb+ had identical actions to Cs+ on the 5-HT-sensitive K+ current. 5. The Ba2+-, Rb+- and Cs+-sensitive component of the 5-HT-sensitive K+ current inwardly rectified with a reversal potential that was dependent on the K+ equilibrium potential (EK). 6. Replacing external Na+ with N-methyl-D-glucamine, blocking Ca2+ entry, or preventing an increase in intracellular [Ca2+] with BAPTA, all failed to alter I5-HT at potentials depolarized to EK. 7. I5-HT at depolarized potentials was reversibly blocked by 4-aminopyridine (4 mM) but not tetraethylammonium chloride (30 mM) and did not show inactivation during depolarizing voltage pulses (1.5 s duration). 8. The results suggest that, in addition to enhancing Ih, 5-HT modulates two distinct K+ conductances in neonatal rat facial motoneurones. The actions of Cs+, Ba2+ and Rb+ support the involvement of a member of the inwardly rectifying family of K+ channels while the other K+ channel may belong to the voltage-gated family.