源自有症状携带者和健康携带者的人芽囊原虫患者分离株的同工酶模式。
Isoenzyme patterns of Blastocystis hominis patient isolates derived from symptomatic and healthy carriers.
作者信息
Gericke A S, Burchard G D, Knobloch J, Walderich B
机构信息
Institut für Tropenmedizin der Universität, Tübingen, Germany.
出版信息
Trop Med Int Health. 1997 Mar;2(3):245-53. doi: 10.1046/j.1365-3156.1997.d01-258.x.
Isolates of Blastocystis hominis derived from patients with intestinal symptoms and from healthy carriers were cultured in vitro and isoenzyme patterns of hexokinase (E.C. 2.7.1.1), phosphoglucomutase (E.C. 2.7.5.1) and glucose phosphate isomerase (E.C. 5.3.1.9) were investigated to find evidence for pathogenic and non-pathogenic subspecies of B. hominis. For this purpose we examined 2000 patients of the out-patient department of the Institute for Tropical Medicine in Tübingen. From these, we obtained 232 B. hominis patient isolates; 119 isolates could be tested for the three isoenzymes. Blastocystis hominis possesses 5 patterns for hexokinase, 11 for phosphoglucomutase and 35 for glucose phosphate isomerase, showing that B. hominis is highly polymorphic. However, there was no correlation between isoenzyme patterns and disease of patients.
对从有肠道症状的患者和健康携带者中分离出的人芽囊原虫进行体外培养，并研究己糖激酶（E.C. 2.7.1.1）、磷酸葡萄糖变位酶（E.C. 2.7.5.1）和磷酸葡萄糖异构酶（E.C. 5.3.1.9）的同工酶模式，以寻找人芽囊原虫致病和非致病亚种的证据。为此，我们检查了图宾根热带医学研究所门诊部的2000名患者。从中，我们获得了232株人芽囊原虫患者分离株；119株分离株可用于检测这三种同工酶。人芽囊原虫的己糖激酶有5种模式，磷酸葡萄糖变位酶有11种模式，磷酸葡萄糖异构酶有35种模式，表明人芽囊原虫具有高度多态性。然而，同工酶模式与患者疾病之间没有相关性。
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