Ambili M, Pillai M R, Sudhakaran P R
Department of Biochemistry, University of Kerala, Kariavattom, Trivandrum, India.
Indian J Biochem Biophys. 1997 Aug;34(4):347-53.
In order to study the role of matrix degrading enzymes in modulating cell matrix interaction, an understanding of the characteristics and regulation of their activity is useful. A number of matrix degrading metalloproteinases are involved in modulating the cell-ECM interactions during the involutory phase of mammary gland resulting in its remodelling. Zymographic studies showed that three types of gelatinases (60K, 68K and 130K) occur during the different phases of involution. The 60K gelatinase which appeared on the fifth day of involution has been purified by affinity chromatography over gelatin sepharose. Zymographic and radiolabelled substrate digestion studies at different pH and in presence of different cations showed that the activated form of this gelatinase is a Ca2+ dependent neutral matrix metalloproteinase capable of cleaving collagen I and collagen IV. Immunocytochemical studies showed that the enzyme is localised at pericellular/extracellular sites.
为了研究基质降解酶在调节细胞与基质相互作用中的作用，了解其活性特征和调控机制是很有用的。许多基质降解金属蛋白酶参与调节乳腺退化期的细胞与细胞外基质相互作用，从而导致其重塑。酶谱分析表明，在退化的不同阶段出现三种类型的明胶酶（60K、68K和130K）。在退化第5天出现的60K明胶酶已通过在琼脂糖明胶上进行亲和层析纯化。在不同pH值和不同阳离子存在下进行的酶谱分析和放射性标记底物消化研究表明，这种明胶酶的活化形式是一种依赖Ca2+的中性基质金属蛋白酶，能够切割I型胶原和IV型胶原。免疫细胞化学研究表明，该酶定位于细胞周围/细胞外位点。
