Sharma M, Joshi P G, Joshi N B
Department of Biophysics, National Institute of Mental Health and Neuro Sciences, Bangalore, India.
Indian J Biochem Biophys. 1997 Aug;34(4):379-84.
Mechanism of merocyanine 540 (MC540) mediated photosensitization in glioblastoma (U-87MG) and neuroblastoma (Neuro 2a) cells was investigated. Photoinduced lipid peroxidation was measured in the presence of mechanistic probes-deuterium oxide (D2O), sodium azide, superoxide dismutase (SOD), mannitol and sodium benzoate. In both the types of cells, the photoinduced lipid peroxidation was enhanced in D2O whereas it showed inhibition in the presence of sodium azide. SOD also inhibited the lipid peroxidation while sodium benzoate and mannitol had no effect. These results suggest that photosensitization of U-87MG and Neuro 2a cells by MC 540 involves both type I (free radical mediated) and type II (singlet oxygen mediated) mechanisms.
研究了部花青540（MC540）介导的胶质母细胞瘤（U-87MG）和神经母细胞瘤（Neuro 2a）细胞光致敏作用的机制。在存在机制探针——重水（D2O）、叠氮化钠、超氧化物歧化酶（SOD）、甘露醇和苯甲酸钠的情况下测量光诱导的脂质过氧化。在这两种类型的细胞中，光诱导的脂质过氧化在重水中增强，而在叠氮化钠存在时表现出抑制作用。超氧化物歧化酶也抑制脂质过氧化，而苯甲酸钠和甘露醇没有作用。这些结果表明，MC 540对U-87MG和Neuro 2a细胞的光致敏作用涉及I型（自由基介导）和II型（单线态氧介导）机制。
