Sakurai T, Amemiya A, Ishii M, Matsuzaki I, Chemelli R M, Tanaka H, Williams S C, Richardson J A, Kozlowski G P, Wilson S, Arch J R, Buckingham R E, Haynes A C, Carr S A, Annan R S, McNulty D E, Liu W S, Terrett J A, Elshourbagy N A, Bergsma D J, Yanagisawa M
Howard Hughes Medical Institute, Department of Molecular Genetics, University of Texas Southwestern Medical Center at Dallas, 75235-9050, USA.
Cell. 1998 Feb 20;92(4):573-85. doi: 10.1016/s0092-8674(00)80949-6.
The hypothalamus plays a central role in the integrated control of feeding and energy homeostasis. We have identified two novel neuropeptides, both derived from the same precursor by proteolytic processing, that bind and activate two closely related (previously) orphan G protein-coupled receptors. These peptides, termed orexin-A and -B, have no significant structural similarities to known families of regulatory peptides. prepro-orexin mRNA and immunoreactive orexin-A are localized in neurons within and around the lateral and posterior hypothalamus in the adult rat brain. When administered centrally to rats, these peptides stimulate food consumption. prepro-orexin mRNA level is up-regulated upon fasting, suggesting a physiological role for the peptides as mediators in the central feedback mechanism that regulates feeding behavior.
下丘脑在进食和能量平衡的综合控制中起着核心作用。我们已经鉴定出两种新型神经肽，它们都是通过蛋白水解加工从同一前体衍生而来，能结合并激活两个密切相关的（以前）孤儿G蛋白偶联受体。这些肽被称为食欲肽A和B，与已知的调节肽家族没有明显的结构相似性。前食欲肽原mRNA和免疫反应性食欲肽A定位于成年大鼠脑内下丘脑外侧和后部及其周围的神经元中。当向大鼠中枢给药时，这些肽会刺激食物摄取。禁食时前食欲肽原mRNA水平上调，表明这些肽在调节进食行为的中枢反馈机制中作为介质发挥生理作用。
