Chai W, Lawson A M, Gradwell M J, Kogelberg H
The Glycosciences Laboratory, Imperial College School of Medicine, Northwick Park Hospital, Middlesex, England, UK.
Eur J Biochem. 1998 Jan 15;251(1-2):114-21. doi: 10.1046/j.1432-1327.1998.2510114.x.
The structures of two hexasaccharides and an octasaccharide, isolated from shark cartilage chondroitin sulphate C after partial chondroitin ABC lyase digestion, have been elucidated by disaccharide analysis, liquid secondary-ion mass spectrometry, and 1H/13C NMR spectroscopy. In the case of the octasaccharide, the order of the internal disaccharide units was deduced from a novel one-dimensional TOCSY/ROESY excitation sculpting experiment [Gradwell M. J., Kogelberg, H. & Frenkiel, T. A. (1997) J. Magn. Reson. 124, 267-270] which incorporates two double-pulsed field-gradient spin-echo sequences. The oligosaccharides each contain the unusual motif GalNAc(4SO3-)beta1-4GlcA(2SO3-)beta1-3GalNAc(6SO3++ +-). From the adjoining sequences in the oligosaccharides, it is apparent that this motif occurs interspersed with typical chondroitin sulphate-C disaccharides, GlcAbeta1-3GalNAc(6SO3-). Such differentially sulphated domains along the glycosaminoglycan chains are potential sites for specific recognition processes.
从鲨鱼软骨硫酸软骨素C经部分软骨素ABC裂解酶消化后分离得到的两种六糖和一种八糖的结构，已通过二糖分析、液体二次离子质谱和1H/13C核磁共振光谱得以阐明。对于八糖，其内部二糖单元的顺序是通过一种新颖的一维全相关谱/旋转框架Overhauser效应谱激发雕刻实验推导出来的[Gradwell M. J., Kogelberg, H. & Frenkiel, T. A. (1997) J. Magn. Reson. 124, 267 - 270]，该实验结合了两个双脉冲场梯度自旋回波序列。这些寡糖均含有不寻常的基序GalNAc(4SO3-)β1 - 4GlcA(2SO3-)β1 - 3GalNAc(6SO3++ + -)。从寡糖中的相邻序列可以明显看出，该基序与典型的硫酸软骨素C二糖GlcAβ1 - 3GalNAc(6SO3 -)穿插出现。沿着糖胺聚糖链的这种硫酸化差异区域是特异性识别过程的潜在位点。
