Terada Y, Tsuji M, Nakamura Y, Hagiwara K, Takahashi K, Ishihara C
Department of Grazing Animal Production, National Grassland Research Institute, Nishinasuno, Tochigi, Japan.
J Vet Med Sci. 1998 Jan;60(1):115-7. doi: 10.1292/jvms.60.115.
We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms in RBC were stained by 4',6-diamidino-2-phenylindole. With the aid of these techniques the piroplasm transmission from one to another race of Bo-RBCs could successfully be quantified in SCID mice.
