牛促甲状腺激素释放激素受体基因的分子克隆

Molecular cloning of bovine thyrotropin-releasing hormone receptor gene.

作者信息

Takata M, Shimada Y, Ikeda A, Sekikawa K

机构信息

National Institute of Animal Health, Tsukuba, Ibaraki, Japan.

出版信息

J Vet Med Sci. 1998 Jan;60(1):123-7. doi: 10.1292/jvms.60.123.

DOI:10.1292/jvms.60.123

PMID:9492373

Abstract

The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using PCR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaques were screened to obtain several clones each containing the N-terminus or C-terminus domain. The bovine TRHR gene encoded 398 amino acids and has a long intron. The identity of the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. RT-PCR analysis indicated TRHR mRNA to be expressed in the pituitary and brain.

摘要

编码牛促甲状腺激素释放激素受体（TRHR）的基因组DNA是从牛（荷斯坦奶牛）基因组文库中分离得到的。以牛候选TRHR跨膜结构域（TMD-III-IV）的PCR片段和小鼠TRHR cDNA的C末端结构域为探针，筛选了9×10⁵个噬菌斑，获得了几个分别包含N末端或C末端结构域的克隆。牛TRHR基因编码398个氨基酸，并有一个长内含子。推导的牛TRHR氨基酸序列与小鼠、大鼠或人类的氨基酸序列同一性超过88%。逆转录-聚合酶链反应（RT-PCR）分析表明TRHR mRNA在垂体和脑中表达。