Alonso J R, García-Ojeda E, Weruaga E, Briñón J G, Arévalo R, Celio M R, Aijón J
Departamento de Biología Celular y Patología, Universidad de Salamanca, Spain.
Arch Ital Biol. 1998 Mar;136(2):77-81.
The monoclonal antibody McAB 300 against calbindin D-28 k demonstrated an immunostaining pattern in the teleost brain completely different to those obtained in other classes of vertebrates, or after using in the teleost brain other monoclonal and polyclonal antibodies raised against the same protein. Although calbindin D-28k is considered a neuronal marker, McAB 300 specifically stained glial cells throughout the brains of teleosts, with the only exception of some retinal amacrine and horizontal cells.
抗钙结合蛋白D-28k的单克隆抗体McAB 300在硬骨鱼脑中呈现出的免疫染色模式,与在其他脊椎动物类别中所获得的模式完全不同,也与在硬骨鱼脑中使用针对同一蛋白质产生的其他单克隆和多克隆抗体后所获得的模式不同。尽管钙结合蛋白D-28k被认为是一种神经元标记物,但McAB 300在硬骨鱼的整个大脑中特异性地标记了神经胶质细胞,唯一的例外是一些视网膜无长突细胞和水平细胞。