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猪有齿食道口线虫和四刺食道口线虫的遗传多样性及其同工酶分析鉴定

Genetic diversity in porcine Oesophagostomum dentatum and O. quadrispinulatum and their delineation by isoenzyme analysis.

作者信息

Snábel V, Várady M, Christensen C M, Bjørn H, Nansen P, Corba J, Dubinský P

机构信息

Danish Center for Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Parasitol Res. 1998;84(2):112-6. doi: 10.1007/s004360050366.

DOI:10.1007/s004360050366
PMID:9493209
Abstract

The genetic diversity in eight strains of Oesophagostomum dentatum and O. quadrispinulatum was investigated by the electrophoresis study of ten enzyme systems. The loci Idh-2, Fbp, Sdh, and Pgm were found to be diagnostic between the species examined. Both the proportion of fixed allelic differences (26.3%) and the genetic distance coefficient (D = 0.54) are well above the range for differentiation of valid species. Isoenzyme patterns of susceptible and resistant lines of O. dentatum showed at polymorphic loci a reduced genetic heterogeneity in the latter group. No qualitative difference in terms of the presence/absence of alleles was observed among susceptible and resistant isolates with the enzymes studied. The detection of one possible hybrid indicates that introgression in O. dentatum and O. quadrispinuatum may occur.

摘要

通过对十种酶系统的电泳研究,调查了八株具齿食道口线虫和四刺食道口线虫的遗传多样性。发现Idh - 2、Fbp、Sdh和Pgm基因座在被检查的物种之间具有诊断性。固定等位基因差异的比例(26.3%)和遗传距离系数(D = 0.54)均远高于有效物种分化的范围。具齿食道口线虫敏感系和抗性系的同工酶模式显示,在多态性基因座上,后一组的遗传异质性降低。在所研究的酶中,敏感和抗性分离株之间在等位基因的存在/缺失方面未观察到质的差异。检测到一个可能的杂交种表明具齿食道口线虫和四刺食道口线虫可能发生基因渗入。

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