从部分消化的弹性蛋白中去除吸附的弹性蛋白酶。
Removal of adsorbed elastase from partially digested elastin.
作者信息
Fromageot H P, Slusarczuk G M
出版信息
Biochem J. 1976 Jun 1;155(3):705-7. doi: 10.1042/bj1550705.
Abstract
Bovine ligamentum nuchae elastin that had been partially digested by porcine pancreatic elastase and then thoroughly washed with 0.2 M-NaC1/0.05 M-sodium borate solution continued to release peptide fragments in solution. Complete removal of elastase from the elastin surface required extraction with dilute alkali, a procedure that does not irreversibly inactivate the enzyme or hydrolyse elastin.
摘要
经猪胰弹性蛋白酶部分消化后，再用0.2M氯化钠/0.05M硼酸钠溶液充分洗涤的牛项韧带弹性蛋白，在溶液中持续释放肽片段。从弹性蛋白表面完全去除弹性蛋白酶需要用稀碱提取，该过程不会使酶不可逆地失活或水解弹性蛋白。
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本文引用的文献
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