Clayton T M, Whitaker J P, Sparkes R, Gill P
Forensic Science Service, Wetherby Laboratory, West Yorkshire, UK.
Forensic Sci Int. 1998 Jan 9;91(1):55-70. doi: 10.1016/s0379-0738(97)00175-8.
The use of multiplex PCR and fluorescent dye technology in the automated detection and analysis of short tandem repeat loci provides not only qualitative information about the profile--i.e. which alleles are present--but can also provide quantitative information on the relative intensities of the bands, and is therefore a measure of the amount of amplified DNA. The availability of this quantitative information allows for the interpretation of mixtures in a detailed way which has not been previously possible with many other human identification systems. In this paper we present a simple approach to the resolution and analysis of mixed STR profiles resulting from the testing of mixed biological stains in forensic casework and highlight factors which can affect it. This approach requires a detailed knowledge--gained through a mixture of experiments and validation studies--of the behaviour of each locus within the multiplex systems described. We summarise the available data from previously published experimental work and validation studies to examine the general principles underlying this approach.
多重聚合酶链反应（PCR）和荧光染料技术在短串联重复序列（STR）位点的自动检测与分析中的应用，不仅能提供有关图谱的定性信息——即存在哪些等位基因，还能提供条带相对强度的定量信息，因此可作为扩增DNA量的一种度量。这种定量信息的可用性使得能够以一种详细的方式解释混合样本，而这在许多其他人身份识别系统中以前是无法做到的。在本文中，我们提出了一种简单的方法来解析和分析法医案件工作中混合生物污渍检测产生的混合STR图谱，并强调了可能影响它的因素。这种方法需要通过实验和验证研究相结合获得的关于所描述的多重系统中每个位点行为的详细知识。我们总结了先前发表的实验工作和验证研究中的可用数据，以检验这种方法的一般原理。
