Coniglio L, Morale A, Angelini C, Falugi C
Institute of Comparative Anatomy, University of Genoa, Italy.
J Exp Zool. 1998 Mar 1;280(4):314-20. doi: 10.1002/(sici)1097-010x(19980301)280:4<314::aid-jez5>3.0.co;2-r.
During metamorphosis, ascidian tadpoles settle on appropriate substrates, by secreting adhesive substances from the papillae. The papillae were shown by different authors to possess neural structures, probably for substrate recognition. In free-swimming tadpoles, AChE activity was localized by histochemical methods at the cell surface of the adhesive papillae, suggesting the possible presence of a cholinergic system in these structures. In vivo experiments showed that the treatment with AChCl caused dose-dependent enhancement of metamorphosis, shortening of the exploratory period of the larvae, and secretion within a few minutes of sConA-binding glycoconjugates in the tunica, beginning from the adhesive papillae.
在变态过程中，海鞘蝌蚪通过从乳头分泌粘性物质附着在合适的基质上。不同作者已表明乳头具有神经结构，可能用于识别基质。在自由游动的蝌蚪中，通过组织化学方法将乙酰胆碱酯酶（AChE）活性定位在粘性乳头的细胞表面，这表明这些结构中可能存在胆碱能系统。体内实验表明，用氯化乙酰胆碱（AChCl）处理会导致变态的剂量依赖性增强、幼虫探索期缩短，并且从粘性乳头开始，在几分钟内被囊分泌伴刀豆球蛋白A（sConA）结合糖缀合物。
